FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_29.35100000043aec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_29.35100000043aec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187138
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC12451466.53592535989483No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT2209011.80412316044844No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG73893.948423088843527No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCAGAATCTCGTATGCCGTC1980.10580427278265238RNA PCR Primer, Index 40 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTAG406.7630026E-944.99973336
CTTTATA207.018343E-444.9997332
GTATACC253.8783888E-544.99973342
AGTATGC406.7630026E-944.99973341
TACCGTC253.8783888E-544.99973345
ATTCGTA207.018343E-444.99973337
GAATCTA406.7630026E-944.99973335
GAATTCG207.018343E-444.99973335
TCGTATA253.8783888E-544.99973340
TGCCGTC130800.044.41487545
CTAGTAT501.0713848E-940.4997639
ATCTAGT451.9135769E-839.99976337
ATGCCGT153800.037.743644
TATACCG301.1363122E-437.4997843
CGTATAC301.1363122E-437.4997841
TCTAGTA552.7230271E-936.81796338
GAGCCCA161250.036.1672316
AGCCCAC161250.036.1393217
GCCCACG161200.036.1365718
CCCACGA161200.036.1365719
Produced by FastQC (version 0.11.2)