FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_28.35100000043ac2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_28.35100000043ac2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445659
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC202694.5480961901364045No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT42820.9608243073740237No Hit
ATATACAGCCTGTTGAATCAAATAGACAGGTATGAGCCAACCTAGCGAACT13030.2923760094601478No Hit
ACATAAAAGCAAAACTATAGGCTGTCTTTACTTAGCAAATAAGCAGACTAG9100.2041919943275015No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC8910.19992864499538887No Hit
GGATACAGCTAGTAGCTTTCCACTTTCGACTACAGGACTTTTACCTTCTTT7710.17300222816099303No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG6830.1532561891491028No Hit
GGTAATTTTGGCTTAACGTGTTTAGAGCTTTTCTTGGAAGCTTGATTGTTT5840.13104189526072624No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC5800.13014434803291305No Hit
AGATAAATCTATCGGTTTAGGCTCTTCCCCTTTCGCTCACCACTACTTGGG5650.1267785459286136No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5580.1252078382799405No Hit
ACTTGGGGAATCTCGTTTGATTTCTTTTCCTCTAGCTACTAAGATGTTTCA5380.12072010214087452No Hit
CCGATATACAGCCTGTTGAATCAAATAGACAGGTATGAGCCAACCTAGCGA4890.1097251486001629No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4850.10882760137234972No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4760.10680812010977003No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4700.10546179926805023No Hit
CCCGATATACAGCCTGTTGAATCAAATAGACAGGTATGAGCCAACCTAGCG4670.10478863884719034No Hit
CTACTAAGATGTTTCAGTTCGCTAGGTTGGCTCATACCTGTCTATTTGATT4620.10366670481242385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC21200.043.62017445
TATCACG501.0786607E-940.49992
ATCACGC451.9237632E-839.99993
GCCGTCT552.741217E-936.8180945
CACGCAG504.8676156E-835.9999125
ACGGTGT702.1827873E-1135.357053
ATGCCGT26200.035.2957144
GAATTGG458.6765795E-735.003841
TATGCCG26900.034.3772443
CTCGTAT26750.034.31767339
GCAGAAT26850.034.2736632
GACCTTG26950.034.2299726
CGTATGC26850.034.18985741
TCGTATG26850.034.18985740
ACGAGAC27050.034.18660722
TGCAGAA26950.034.1464831
CAGAATC27100.034.1235333
AGACCTT27200.033.99807425
TTGCAGA27250.033.85312730
GAGACCT27350.033.81161524
Produced by FastQC (version 0.11.2)