FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_27.35100000043aa9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_27.35100000043aa9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410498
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC11424627.831073476606466No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT223785.451427290754157No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG60811.48137140741246No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT12880.31376523149930086No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT10170.24774785748042624No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC10060.24506818547228001No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT9700.23629834980925607No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT9300.2265540879614517No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT9170.2233872028609153No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC9130.22241277667613482No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA8350.20341146607291632No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA8310.20243703988813586No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC8030.1956160565946728No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC7860.19147474530935596No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT7850.19123113876316086No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC7530.18343572928491736No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC7010.17076818888277168No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCT6990.17028097579038143No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC6530.1590750746654064No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG6450.15712622229584555No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA6200.1510360586409678No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT6120.14908720627140692No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC5870.14299704261652918No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5860.14275343607033408No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT5830.14202261643174874No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5790.1410481902469683No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT5630.13715048550784656No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5610.13666327241545637No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC5600.13641966586926124No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC5490.13373999386111504No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA5340.1300858956681884No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA5200.12667540402145686No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT5150.12545737129048132No Hit
TTTTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA5110.12448294510570088No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4940.12034163382038403No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC4850.11814917490462803No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAACTGTCTCTTATA4740.11546950289648182No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4610.11230261779594541No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4560.11108458506496988No Hit
TTTTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4530.11035376542638453No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA4420.10767409341823835No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATC4410.10743048687204323No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC4360.10621245414106767No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT4260.10377638867911658No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC4200.10231474940194593No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAGA207.0212333E-445.0087744
CGAATCT207.029668E-444.99780734
CATAATC207.029668E-444.99780733
CCGAATC207.029668E-444.99780733
TGCCGTC117750.044.3535645
GCCGTCT1600.039.37787645
GACCTTT356.240565E-638.5695526
ATGCCGT141600.036.867144
GGGTATT250.00210603835.99824519
GCATAAT250.00210603835.99824532
AATCTCG147650.035.19977636
ATCTCGT147750.035.19117737
CTCGTAT147900.035.18591339
GAATCTC147700.035.15739435
CAGAATC147850.035.1521633
TCTCGTA148050.035.1350738
TATGCCG148500.035.1346543
GTATGCC148250.035.13319442
AGAATCT147550.035.1321434
TCGTATG148050.035.11987340
Produced by FastQC (version 0.11.2)