FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_25.35100000043a59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_25.35100000043a59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656964
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC93301.420169141688129No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT19430.29575440967846034No Hit
CCATATGACTTAGCCATTTTCAGTTTGGGATATTGTTATCCAAAGAACCAA13730.2089916646878672No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6740.1025931405678241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10200.042.57201845
ATGCCGT12050.036.03606444
CATCGCG250.0021068235.99872234
TATGCCG12400.035.20036343
CGTATGC12700.034.54601741
GTATGCC12700.034.3688642
CTCGTAT12800.034.1003539
TCAGCCC401.5599328E-533.748814
CGAGACC12900.033.66415823
TCGTATG13050.033.44708640
ACCTTGC13100.032.9784427
ACGAGAC13350.032.69795622
ATCTCGT13350.032.6954737
AATCTCG13350.032.6954736
CCTTGCA13250.032.60509528
CACGAGA13400.032.5759521
GCAGAAT13350.032.5294132
CCCACGA13450.032.4548519
CTTGCAG13450.032.4548529
TCTCGTA13700.032.0244138
Produced by FastQC (version 0.11.2)