FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_24.35100000043a3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_24.35100000043a3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159427
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC133048.34488511983541No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT35022.1966166333180706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG5940.3725843175873597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13300.043.47532345
GAGCCGA250.002098946836.009546
AGCCGAT250.00210218335.9982457
ATGCCGT16450.035.1502644
GCCGTCT401.5527005E-533.74835245
CGTATGC17150.033.58436641
TATGCCG17150.033.58436643
CCACGAG17350.033.3269120
CACGAGA17450.033.26485421
ACGAGAC17450.033.26485422
TCGTATG17250.033.25924740
ATCTCGT17200.033.2251237
TCCTATC17350.033.1972333
CGAGACT17500.033.1698123
AGATCCT17300.033.1631230
GATCCTA17300.033.1631231
CCCACGA17450.033.1359219
GTATGCC17400.033.10183342
GAGCCCA17400.033.10183316
AGACTAG17550.033.0753125
Produced by FastQC (version 0.11.2)