FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_23.35100000043a15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_23.35100000043a15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191184
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC40262.1058247552096407No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT11150.5832077998158842No Hit
TTGTAGTACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA2710.14174826345300862No Hit
GTTACAAAAACAGATTCGGACTCTATTACGTCGATTTCAAAAATAACCTCA2280.11925684157670098No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2070.10827265879989956No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG207.019124E-444.9992149
TGCCGTC4150.043.37273845
CGCGCGC301.13648195E-437.49934410
ATGCCGT4950.036.36300344
GACCGTC250.002102906135.999377
CCGGACA250.002102906135.9993735
GCACCGG250.002102906135.9993732
GGCGCAC250.002102906135.9993729
TATGCCG5250.034.2851243
TATCTCG5250.034.2851236
CGTATGC5300.033.96167441
CTCGTAT5300.033.96167439
GTATGCC5300.033.96167442
TCGTATG5400.033.33275240
CCTATCT5400.033.33275234
CTAGATC5350.032.80316528
GCCCACG5500.032.726718
CCCACGA5500.032.726719
TCTCGTA5500.032.726738
CGAGACT5500.032.726723
Produced by FastQC (version 0.11.2)