FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_19.351000000439aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_19.351000000439aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162491
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC64733.983605245829No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT25031.5403930063818918No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG4170.2566295979469632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6900.043.3612645
GCATAAG250.002100596736.004195
ATGCCGT9800.030.52986544
GCTCTAT453.4625627E-530.0127281
TAAGGGT300.005131485430.0034894
GACGCAT300.005131485430.0034892
TAATGCA300.005131485430.0034894
TATGCCG10250.029.18953143
CGTATGC10200.029.11207241
GTATGCC10350.028.90750742
TCCTATC10450.028.85503233
TATCTCG10300.028.82942836
CTCGTAT10350.028.69015739
TCGTATG10350.028.69015740
CCTATCT10550.028.58152434
ATCCTAT10500.028.50331532
TAGATCC10500.028.50331529
CGAGACT10550.028.36822923
CTAGATC10600.028.23441528
TCTCGTA10550.028.14626538
Produced by FastQC (version 0.11.2)