FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_16.3510000004394d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_16.3510000004394d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73108
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC791310.82371286316135TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT13211.8069157958089401No Hit
GACAGAAACTATATACCAAAACAAAACTCCTGATTTTTCCTCAATCTTAAT3410.4664332220823986No Hit
GAAAAAAAAATCGAATCGAAATTAAAAGAATCGAATCGAATCAAAGCATAA3030.41445532636647153No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG2450.3351206434316354No Hit
TAGTGACATAAAACTTCAAACACTTCTACCAAAATGGAAGGATATTATCAA2410.3296492859878536No Hit
CAACAGATACACAAAAAGCTAGAAATTAAAACGTACCACCAGAAAAAATTA2390.3269136072659627No Hit
GTATATAGACTCTAGAAAGGATGCCAAAGAATTGGTTAAAGGCTTAATGGA1750.23937188816545385No Hit
AATCAGGAGTCTTTTCACAGAGTATTTCAATTGCAAATCAAAGATAGCGAT1700.23253269136072657No Hit
GAGTAGAACAATAACTAAAATAGCCTTGTCATTTCCTTTCTATGCAGTATG1630.22295781583410845No Hit
GTATATACCTTGTGTGAACATTATATGGATTTAGAAGGCATGTATGCACGC1540.21064726158559938No Hit
ATATTGCTAACTCTTCTATGGATGAAGATAATTTTGAAGACTTCAAGGTAC1480.20244022541992668No Hit
ACGGTACATGATATCAACTTCATGTATGATACCAATAAATCGGCAAGTAAA1450.19833670733709033No Hit
TTGTGTTTCTGGTTATACCTCTCATCCATGGTTTATGTTCATGATCTAATT1450.19833670733709033No Hit
TTAGAATCATTTTTCGGACATTTGAAAAACCATATCCAAGTTCATCGAGGA1400.19149751053236308No Hit
ACTTTACATTATGCACAAATTGATGTAGAAAAGTAGCAGTGAAATGAAACG1390.19012967117141763No Hit
GTAATAATGTACAAGGCTTGCATAAATTATCATCGACTGCTAAGTTAGATT1260.17234775947912676No Hit
GAAGAAGAGAGATTGTTCTTTTATCCTTTTCCAGAAAGATCAGCATACAGG1250.17097992011818133No Hit
CTTACGTTTTGTTTCTACCAATCTTCCTTCACTTAATATCAATGCATGAGC1240.16961208075723588No Hit
CTGTAATAATTGCCATTTGTTAAGATTGTTAAGTTGAAACAAATTGTTGGG1240.16961208075723588No Hit
ATATACAAGTGAATAAAGCAGATGAAATCTTTGCTCTCATGGAATTTGCAT1230.16824424139629043No Hit
TAATTCAACAAGTTCATACGGTTGATATTGTTCTTCTAAAGATGATGTTTC1220.16687640203534498No Hit
GGAAAGATTACTGGAATACGGACTATTGAGCATTTGTAATTAGCTTTGAAC1200.16414072331345406No Hit
AATCTGTAGTTGTCTATTGTCTAATCTGCTTTCTAGATCTTTCATCCAATC1180.16140504459156316No Hit
GTATATTAACAAAAACAGCCAACATTTTAATCCAACTGTATCTCTTAAGTG1160.15866936586967229No Hit
ATTTTCAAAAAGCTAGAAGAGAAGATTTTGAATGTTGTCAACACAAAAGTA1140.15593368714778136No Hit
ACATGTTTTATACCTTCATACTCTTTCCTTGTGATAACTTGTTTCAGTCTC1110.151830169064945No Hit
ATATAAAGCAAACATTGGCATATCTGAAGGGAGAAATAGCAATACAGTAAT1100.15046232970399956No Hit
ACATAAATGTCTAGAAAATGCTCTCCTTTCTAAAAATGTATTCCTCTACAG1070.1463588116211632No Hit
ATATTATGTGTTATCTGTCGCATTTGAGAATATTCAGTTCCACTGTATGGG1050.1436231328992723No Hit
ACTTCAAGAAGAGTTAGATAGAGTACGTGATGAAGTATATGAAAATCTTCA1040.14225529353832686No Hit
GAAGGAAGGAATGAAAGAAAAAAGACAAGAAGGAAAGAAAGAAAAGACAAA1030.1408874541773814No Hit
GTATTCAAACAAAAAGCGTGTATATGAATGTTCATAGTAGCACTGTTCACA1030.1408874541773814No Hit
GAACATCCTATCATCAAATACATCACTGAGTTCATTTTTAAGAAAGAAGCA1000.13678393609454506No Hit
ATCTGGAACTCTATTCAAATGCATGTTTGACTTTTTCTTCCTAGCAATCCA980.13404825737265416No Hit
CAATAAGTCATACACAAAAGATATGACAATTGTTTCTAATGCTTCTTGTAC920.12584122120698146No Hit
CAACCAAAACTTCTAACGGATTGAGTAAGGTGTAAACAATGATTTTTGGAA910.12447338184603601No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC900.12310554248509055TruSeq Adapter, Index 12 (95% over 24bp)
AGATAAATAAGATACATTCATGGTAGCAGCTTGCAAAAAATAGCCAGTAAT890.1217377031241451No Hit
ATGATAGAAAAGGAAACATCTTCATGGAAAACATAGATAGAATCATTCTCA880.12036986376319965No Hit
GTATGTTTCATTGTAGAATGTGCTAAGTATTGCCATTGATGAATTAATTGA860.11763418504130874No Hit
GGTAAATAAAAGAAACATTTAAACTGCCTTTCCTTTCCTGAACAAAATAAA840.11489850631941785No Hit
TCTACTACATCTTCTTTCTTCTCTTCTTTGTTATCAAACGTGATGTTTTCA830.1135306669584724No Hit
AATATGTACACCCATTGAAACAGCTTCTTCTATTTCGCTAAAGTGAATACC820.11216282759752695No Hit
ATATTAAACCATCTGAACTTCGAGGTGTGAAATCTAATGGGATGATTTGCT810.1107949882365815No Hit
TTATACACGTATTCTCAGATCAAAGATTTAGAACGAACAAAAGTTCATACT780.10669147015374514No Hit
CTTCTATCTTTCACTTCAAGTAAAATCTGAGCATTGGTTCTTTGGTGATTT770.10532363079279969No Hit
GTATAGTCAGTAAGGGGCTACTAATGATAAATTCCCTTGCGTTTGAAGAAT770.10532363079279969No Hit
AAAATGATACTTAGATTGGAAATGAACCATAATTTTCAAACCAATCTCAAA750.1025879520709088No Hit
ATCTGAATCTACTCTTTTTTTTTTTCTTAGTCTGGCTAACGGTTTGTCAAT750.1025879520709088No Hit
ATCCTAACTTTTTCAATGTCCTTGAGCAAAACACTTAAATATTCTGTGCCT740.10122011270996334No Hit
TAAATAGGAGATGTGATTTTGTTAGGAATTATGGAAAACAAGGTGAGAACT740.10122011270996334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAC253.839726E-545.0301171
TAGACTC206.990499E-444.9993176
TATATAG206.990499E-444.9993172
ATATAGC206.990499E-444.9993174
CATACGG206.990499E-444.99931715
GGTGCAC206.990499E-444.99931736
GCTACAC206.990499E-444.9993179
CAACTGC206.990499E-444.99931715
TGGTGCA206.990499E-444.99931735
TGCAGGA206.990499E-444.99931720
TTCCTAG206.990499E-444.99931738
AAGTAAG253.855298E-544.99931339
GGTACAT253.855298E-544.9993133
TGGACTG253.855298E-544.99931325
CTAACTC302.1378546E-644.9993137
TATTATC302.1378546E-644.99931343
CGTACCA253.855298E-544.99931332
TATAGAC302.1378546E-644.9993134
ACGTACC253.855298E-544.99931331
CGGTACA253.855298E-544.9993132
Produced by FastQC (version 0.11.2)