FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_14.35100000043909.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_14.35100000043909.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179601
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC4120022.93973864288061TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT1979011.018869605403088No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG24051.339079403789511No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCC207.018666E-444.9983343
CGAGTTG207.018666E-444.9983318
GTCTCGA302.155799E-644.9983339
CCAATCG207.018666E-444.9983331
TGGTTAG207.018666E-444.9983336
TGTCTCG253.878479E-544.9983338
TCGAGAC207.018666E-444.9983335
CACTTGC207.018666E-444.9983339
TGCCGTC44200.044.489345
GCCGTCT403.4375444E-739.37353545
TACCTGC250.002099898636.0086866
TCACTAG250.002099898636.0086863
TGATACG250.002102771835.9986623
ACAGTCC250.002102771835.998668
GTAATCT250.002102771835.9986635
CGAGACC250.002102771835.9986636
TAGGCCT250.002102771835.9986644
GTAGTCA250.002102771835.9986632
ATCGAGA250.002102771835.9986634
CGCTTCT250.002102771835.9986628
Produced by FastQC (version 0.11.2)