FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0113_pa_11.351000000438ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0113_pa_11.351000000438ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269284
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC56692.1052123408743184TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT11020.4092333744299698No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC9860.36615617712155196No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5170.19199061214182797No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4750.17639369587498702No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4310.16005406930972504No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3730.13851547065551612No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3580.13294514341735864No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT3380.12551804043314865No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC3370.12514668528393816No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA2860.10620757267420271No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6300.041.06939745
ATGCCGT6800.038.04958744
GTATGCC7000.036.96245642
CGTATGC7050.036.7003141
TATGCCG7050.036.7003143
AATCTCG7250.036.30854836
TACTTGC7100.036.12497329
CTCGTAT7350.036.1206639
ATCTCGT7300.036.0598637
CGTATTG250.002104761535.998228
CCACTAC250.002104761535.998229
ACTGTAG250.002104761535.998228
CGAGACT7150.035.8723523
GAGACTA7300.035.4434524
TCGTATG7300.035.4434540
TGCAATC7250.035.3775633
TCTCGTA7400.035.26852438
GACTACT7350.035.2023426
CACGAGA7300.035.13524621
AGACTAC7400.034.96448525
Produced by FastQC (version 0.11.2)