Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0113_pa_1.3510000004375c.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 467526 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC | 3876 | 0.8290448017864247 | RNA PCR Primer, Index 27 (95% over 21bp) |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1422 | 0.30415420746653665 | No Hit |
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC | 1192 | 0.25495908248953003 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1072 | 0.2292920607623961 | No Hit |
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATG | 952 | 0.20362503903526222 | No Hit |
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT | 905 | 0.19357212219213477 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 799 | 0.1708995863331665 | No Hit |
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC | 718 | 0.15357434666735112 | No Hit |
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA | 663 | 0.14181029504241474 | No Hit |
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA | 594 | 0.12705175754931275 | No Hit |
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCC | 560 | 0.11977943472662483 | No Hit |
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGA | 559 | 0.1195655428788987 | No Hit |
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATT | 552 | 0.1180682999448159 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT | 544 | 0.11635716516300698 | No Hit |
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCA | 527 | 0.112721003751663 | No Hit |
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA | 496 | 0.10609035647215342 | No Hit |
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGC | 471 | 0.10074306027900053 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 390 | 0.0 | 39.80306 | 45 |
ATGCCGT | 430 | 0.0 | 36.100445 | 44 |
TATCTCG | 445 | 0.0 | 34.883575 | 36 |
GCTATCT | 450 | 0.0 | 34.507053 | 34 |
ATTGCTA | 450 | 0.0 | 34.507053 | 31 |
ACGAATT | 455 | 0.0 | 34.124207 | 27 |
ATCTCGT | 460 | 0.0 | 33.74607 | 37 |
CCCACGA | 465 | 0.0 | 33.38678 | 19 |
GTATGCC | 465 | 0.0 | 33.38321 | 42 |
TATGCCG | 470 | 0.0 | 33.02807 | 43 |
TCGTATG | 470 | 0.0 | 33.02807 | 40 |
GGTATCA | 2085 | 0.0 | 32.927444 | 1 |
CGAATTG | 475 | 0.0 | 32.69089 | 28 |
TGCTATC | 475 | 0.0 | 32.69089 | 33 |
CTCGTAT | 475 | 0.0 | 32.680405 | 39 |
TTGCTAT | 480 | 0.0 | 32.350365 | 32 |
GAGACGA | 480 | 0.0 | 32.343445 | 24 |
CCGAGCC | 480 | 0.0 | 32.339985 | 14 |
CTATCTC | 490 | 0.0 | 31.686762 | 35 |
CCACGAG | 490 | 0.0 | 31.683372 | 20 |