FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_wt4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_wt4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10382184
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG249280.24010362366916246No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC245140.2361160233723463No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA221770.21360630865336233No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGG206820.19920664091485954No Hit
CTTTTCTGCTTAACGGCCCGCCAACCCTGGAAACGGTTCAGCCGGAGGTA191410.1843639064767105No Hit
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC165220.15913800025119954No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG147470.14204140477571964No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG142640.13738920442943411No Hit
ATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCG141400.13619485071734425No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC134700.12974148791814902No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT133180.12827744143236144No Hit
CTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCC133100.1282003863541621No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA132810.12792106169568945No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGC128800.12405867590094724No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTC124760.12016739445188027No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA124630.12004217994980632No Hit
CTCAAACTTCCGTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCTAGCTG120590.11615089850073934No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC118410.11405114761980716No Hit
CCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCAG114990.1107570430267851No Hit
GCCAGAGGCGCGCGGTGACCGGCTGCGCCGACGACGGCTGCCGGGGGCGC114900.11067035606381086No Hit
CGTTATTTTACTTATTCCGTGAGTCGGAAGCGGGGCCTGGCCCCTCCTTT112780.10862839649152817No Hit
GTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGA110670.10659606880402044No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA105720.10182828584043588No Hit
ATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGC104840.10098067998024307No Hit
GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGAGTCGGAA104300.1004605582023975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG18750.027.815423
GTCCGTT5750.027.3920381
TTACGAC21550.024.2015845
GTTACGA21750.024.1397424
TACGACT24100.022.3670396
TCGCGTT19100.021.6240352
CATGCGT87000.021.5642137
CGCATAG42100.021.28220765
ACATGCG88850.021.273616
ATGCGTG89200.021.1501798
TAAGTCG54850.021.1219089
TGCGTGC92700.020.5022689
TGCGAGT93550.020.31529613
CTCGCGT22600.020.2881571
TTAAGTC57400.020.0619968
CGCGGTT9700.019.8458941
GTGCGAG95450.019.8375712
CTGACAT95550.019.8183363
GACATGC97150.019.8163955
TAGTCCC45650.019.78109228
Produced by FastQC (version 0.11.5)