FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_wt1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_wt1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9356462
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC159900.17089793129069514No Hit
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG111330.1189872838686247No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG110060.11762993319483368No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC98570.1053496503272284No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA97580.10429155806970626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT12900.019.5354651
TGTTACG14250.019.404713
GTTACGA14700.019.0487964
TCGGATG37250.017.3833472
TTACGAC16250.017.2321115
GTCCGTT6000.016.917171
CGCTATG50250.016.85568229
GGGCTTT48800.016.85428860
GTCGCTA51050.016.52306627
GCAACGT52600.016.36904522
TAAGTCG49050.016.3408689
GTGCGAC22500.016.3343433
GCCCGCA77600.016.28252811
AACGCTT52750.016.18927836
GACTGTC36950.016.009114
CATGCGT51500.015.6996657
ATGCGTG51950.015.6984218
ACGTTAA21800.015.5731132
CGCATAG25200.015.55535465
GAACGCT54850.015.50564135
Produced by FastQC (version 0.11.5)