FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12877455
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG950649973.82280893235503No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259080.2011888218595988No Hit
GGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258840.20100244962999286No Hit
GGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239830.18624021594329004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG15600.036.745515
CCAGTCA11800.036.73458526
CTCCAGT14100.035.70184324
CTGAACT17050.034.44079219
CACGTCT16400.034.1013114
CAGTCAC10900.033.99590727
ACACGTC16700.033.90811513
AGGGGGG56250.033.3181273
GGAGGGG58250.032.3425031
GAGGGGG57850.032.3162882
CACACGT17750.031.90265312
CGTCTGA17900.031.82861716
AACTCCA19350.029.62554222
TGAACTC19750.029.02496520
GCACACG20400.028.10244411
GAACTCC20700.027.86230321
ACTCCAG21400.025.97108723
TCTGAAC22550.025.73009118
AGCACAC22600.025.67625810
TCCAGTC18600.025.56042925
Produced by FastQC (version 0.11.5)