FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ox7.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ox7.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730676
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC171630.19658271593173313No Hit
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG134540.15410032396116863No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG114910.13161638342781246No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC107640.12328942226237692No Hit
ATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCG103840.11893695287741751No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA102800.11774575072995494No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC100950.11562678537148784No Hit
GCCAGAGGCGCGCGGTGACCGGCTGCGCCGACGACGGCTGCCGGGGGCGC100210.11477919922810102No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG96580.1106214455787845No Hit
GTCGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGC94970.10877737302357801No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA93740.10736854740686748No Hit
GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGAGTCGGAA92340.10576500605451399No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA89840.1029015393538828No Hit
CCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCAG89350.10234029988055908No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT87910.1006909430609955No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGG87790.10055349665936521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG13800.025.1101043
GTTACGA15300.022.8772324
TCAGGCG17150.021.6331232
TTACGAC16600.021.2969865
GTCCGTT5300.019.811411
TACGACT18050.019.7801846
CGCGGTT10200.019.5589141
GTGCGAC19350.018.6305751
CGGGCAT8050.017.8261681
CATGCGT52250.017.6184167
ATGCGTG52450.017.617978
TCGGATG36950.017.5240612
GTCAGGC23450.017.3135131
CGGAATA41400.017.07698268
ACATGCG54150.017.0006146
TAAGTCG55450.016.6013779
GAACCGC21450.016.47988568
CGCATAG26150.016.4625165
TGCGTGC57050.016.4425479
CTTGTTA21350.016.3935181
Produced by FastQC (version 0.11.5)