FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ox7.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ox7.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10460118
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC171980.16441497122690205No Hit
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG156180.1493099791034862No Hit
ATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCG120450.11515166463705286No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC117150.1119968245100103No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG110580.10571582462071652No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG109250.10444432844830241No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC108310.10354567701817512No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA107450.10272350656082466No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA107320.10259922498006237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG14400.023.3345553
TCAGGCG20850.020.6478732
GTTACGA16200.020.5257644
GTCCGTT6000.019.8336121
TTACGAC16800.019.5847325
CATGCGT64650.019.5447187
ACATGCG67000.019.2774966
ATGCGTG66150.019.2073548
TGCGTGC69750.018.6173739
GTCAGGC25950.018.4781021
TCGGATG40550.017.9534822
GTGCGAG71750.017.65861712
TGCGAGT71800.017.5975713
GACATGC75650.017.4434155
CTGACAT75300.017.3382243
CGGAATA48750.017.15880668
GAATAAG50200.016.87234570
GAGTCGA77050.016.44378516
AGTCGAC77350.016.3798517
TAAGTCG54050.016.3199
Produced by FastQC (version 0.11.5)