FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ox5.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ox5.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8935124
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC104570.11703251124438788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG12150.017.5735663
TAAGTCG31450.017.1391569
ACTCACG29250.017.11070663
CGGAATA28450.017.09967668
TTCCGAC31500.015.77731658
GTTACGA14050.015.6954244
GACTCAC31850.015.60402662
TTAAGTC34600.015.5791488
ACGTTAA14200.015.52780132
TTACGAC14000.015.5015425
CGTTAAA14200.015.28132833
CATGCGT37250.014.9404147
GTCGCTA31700.014.46427127
ATGCGTG38250.014.367048
TAGCGAC25600.014.35512948
ACATGCG38900.014.2174326
CTTCCGA34000.014.20546657
ATAACGT15400.014.09144329
GTCCCTA35450.014.0207488
CGCGTAT39300.013.982561
Produced by FastQC (version 0.11.5)