FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ox5.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ox5.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9412866
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGC249940.26553017964985376No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAG193110.20515536925735478No Hit
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG172340.18308982620171158No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA170480.1811138074206092No Hit
CTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCC169370.17993457040608035No Hit
CCCGGATCGGCCCGAGGGACTCGGGCCTTAGAGCCAAAAGGAGGGGCCAG157980.16783411131105022No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA156110.1658474687730602No Hit
CTTTTCTGCTTAACGGCCCGCCAACCCTGGAAACGGTTCAGCCGGAGGTA144860.15389574227445713No Hit
GCGGTTATGAGTACGACCGGGCGTGGACGGTACTCGGTCCTCCGGATTTT140170.1489132002941506No Hit
GCCAGAGGCGCGCGGTGACCGGCTGCGCCGACGACGGCTGCCGGGGGCGC139510.14821203233956587No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT138460.14709653786636293No Hit
CTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGG130220.13834256219094163No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG128920.13696147379554752No Hit
CGTTATTTTACTTATTCCGTGAGTCGGAAGCGGGGCCTGGCCCCTCCTTT128790.13682336495600808No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGC128540.13655777103381692No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA125000.1327969610955898No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA123250.13093780364025154No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA122800.1304597345803074No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC120950.12849433955609268No Hit
CCGGAAGCCTGGTTACGGTGCCGAACTGCGCGCTAACCTAGAACCCACAA109830.11668072189702902No Hit
GCCGTTCACATGGAACCTTTCTCCTCTTCGGCCTTCAAAGTTCTCATTTG109280.11609641526820842No Hit
GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGAGTCGGAA108860.11565021747892724No Hit
CCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAG108050.11478969317102782No Hit
CCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCT106410.11304739704145368No Hit
GTCGGATGGGGAGCCCGCAGGCCGTTGCGGCGCAGCGCCCCGAGGGGCGC100550.10682187550529244No Hit
CGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATT100460.10672626169330361No Hit
GCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGCCAGAGGCGCGCGGTGACC100410.10667314290886537No Hit
GTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCCCCCGA99230.10541953959612302No Hit
CCCTTGTCCGTACCAGTTCTGAGTCGGCTGTTCGACGCCCGGGGAAGGCC98730.10488835175174066No Hit
GTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGA97800.10390034236118946No Hit
GCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGT96650.10267861031911005No Hit
CCGGGAGATGCCCAGCTTGAGAATCGGGCGGCCGCGCCGTCCGAATTGTA95690.10165872965789591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT9550.045.812591
GTCCGTT3100.041.7751731
CCTGCCA17000.036.647921
CTGCCAG20150.030.0508142
GTGCGAC19850.028.0366673
TCAGGCG14550.026.9426422
CCCCCTT11800.025.5090711
TGCCAGT24800.024.839953
GTCAGGC16850.024.5109621
TGGTCGG42450.023.91002770
TGTTACG14950.023.1784173
GTCCCAT3500.023.0005381
TGCGACG24400.022.8085164
GCCAGGG8250.022.4853761
CTTGTTA16050.021.8073651
GAATAAG57250.021.45810770
GTTACGA16000.021.4385724
CGGGCAT4900.021.4290731
CCGGCAT9150.021.4212671
GGAATAA59550.020.74687869
Produced by FastQC (version 0.11.5)