FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ChiP-zmm16.shoot-line15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ChiP-zmm16.shoot-line15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8474950
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC2266132.6739154803273175TruSeq Adapter, Index 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189890.22406031893993478No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATG86430.10198290255399736TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT274000.061.58109742
TCGTATG289300.061.30040443
CGTATGC299200.060.76938244
TCTCGTA274300.060.45471241
GTATGCC306550.059.70052745
TATGCCG305850.059.608346
CCGTCTT297600.058.8041850
GCCGTCT302350.057.671349
AAAAGGG301700.057.43524670
ATCTCGT290650.057.10210440
GTCACAT331950.056.90505629
ACATGAG334650.056.55965432
ATGCCGT320550.056.0995347
CAGTCAC336300.056.0340327
TCACATG339400.055.7384630
CACATGA340300.055.56916831
GAGCATC310100.055.50698536
AGTCACA341250.055.4362928
CCAGTCA339650.055.40955726
CGTCTTC310300.055.1905351
Produced by FastQC (version 0.11.5)