FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ChiP-zmm16.shoot-line14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ChiP-zmm16.shoot-line14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10465045
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC933600.8921127429456825TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT122100.056.66962442
CGTATGC129450.056.5612444
TCGTATG130550.055.62890643
ACGGTAG133500.055.4483232
CACGGTA135850.054.4891531
TCTCGTA127550.053.06831441
TATGCCG138200.053.00544746
AGTCACG140400.052.62534728
GTATGCC139700.052.53652245
GGTAGCA140300.052.41163334
CGGTAGC142550.051.7316833
GTAGCAT141550.050.53942535
CCGTCTT145400.049.87591650
TAGCATC138050.049.2347936
CAGTCAC154100.048.0605727
GTCACGG154450.048.01941729
GCCGTCT151600.047.8823149
TCACGGT156650.047.2109730
ATGCCGT156250.046.83745647
CCAGTCA158900.046.52110726
Produced by FastQC (version 0.11.5)