FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ChiP-zmm16.shoot-line12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ChiP-zmm16.shoot-line12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9921625
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1547551.5597747344814987TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC204000.060.15066546
ACGTAAT206550.059.13694837
TACGTAA208900.059.091636
CTCGTAT194600.059.08146344
TATGCCG208850.058.90464848
TCGTATG208100.058.86466245
CCGTACG210000.058.815433
GTACGTA211450.058.54449535
CGTAATC195650.058.4423938
CACCGTA212200.058.13965631
ACCGTAC211950.058.12566432
ATGCCGT212150.057.74091749
GCCGTCT211450.057.45292751
GTATGCC215400.057.1134447
CCGTCTT213000.056.82122852
CGTACGT218900.056.4240934
TCTCGTA203700.055.84072543
AATCTCG207600.054.75797341
TGCCGTC222700.054.7077850
ATCTCGT211050.053.96235342
Produced by FastQC (version 0.11.5)