FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ChiP-zmm16.root-line15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ChiP-zmm16.root-line15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8646780
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT527770.6103659396908444TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT72450.052.75255644
CGTATGC75800.051.85251246
TCGTATG76900.051.11079845
TATGCCG77600.051.0108648
TATCTCG76500.049.8225241
CACACTG83900.047.93109531
ACACTGA85250.047.37761732
TCTCGTA81350.047.11029443
GTATGCC84200.046.84584847
ACTGATA86300.046.47673434
ATGCCGT85650.045.97131349
CACTGAT88950.045.3281833
CAGTCAC91450.044.39597327
GTCACAC91700.044.27493329
CCGTCTT88050.044.2020352
GCCGTCT90200.042.99322551
CTGATAT93400.042.98117435
TCACACT94600.042.9176730
CCAGTCA95550.042.4912126
AGTCACA96500.042.2540128
Produced by FastQC (version 0.11.5)