FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ChiP-zmm16.root-line14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ChiP-zmm16.root-line14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8475855
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT333130.3930340950853926TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG199950.2359054042335552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC50200.049.640746
CTCGTAT49400.049.2401644
TCGTATG52600.047.1761145
TATCTCG52050.046.93494841
AGTCACG56650.045.04011528
TCTCGTA54400.044.9717643
TATGCCG55900.044.4542548
ACGAGTG58950.043.69740732
TCACGAG59150.043.4915130
GTCACGA61100.041.9316329
CACGAGT61350.041.93092331
GTATGCC60800.040.92867347
GGATATC60500.040.84237338
GTGGATA63550.040.14889536
ATGCCGT64450.038.55690849
CAGTCAC67700.037.63718827
CGAGTGG69150.037.1505733
CCGTCTT65900.037.018152
GCCGTCT66400.036.7920651
GATATCT68650.036.1465939
Produced by FastQC (version 0.11.5)