FastQCFastQC Report
Wed 24 Jun 2020
H22C3BGXG_n01_ChiP-zmm16.root-line12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH22C3BGXG_n01_ChiP-zmm16.root-line12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7704392
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1065201.382588009540532TruSeq Adapter, Index 21 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338530.4393987221833988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC145250.058.96280346
ACGTTTC154200.058.5365732
CTCGTAT133350.058.50289544
CACGTTT154450.058.44182231
GTCACGT154700.058.3488929
AGTCACG155500.058.18375828
TCGTATG144450.057.76290545
TCACGTT158050.057.11213730
TATGCCG151650.056.98253648
CGTTTCG159550.056.57373433
ACGTCTG165150.055.37738815
CAGTCAC164250.055.25499727
GTTTCGG163100.055.21360834
GTATGCC156900.054.9862747
ATGCCGT155250.054.939849
TCTCGTA140950.054.80214743
GCCGTCT149250.054.428251
CACGTCT168450.054.3756314
ACACGTC169350.054.19069713
CGGAATC150800.054.0772938
Produced by FastQC (version 0.11.5)