FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n02_vk13-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n02_vk13-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201805
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC71286132.37620951900827Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC278771.2660975881152055Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC178690.8115614234684725Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC98390.446860643880816Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC96120.43655092072186225Illumina Single End PCR Primer 1 (98% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC95350.433053789958693Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC93670.4254236864754145Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC89370.40589425494083264Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC76640.34807805414194265Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC66400.3015707567200547Illumina Single End PCR Primer 1 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63710.2893535076902814No Hit
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC62110.2820867424681114Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC56950.25865142462661317Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCC55720.25306509886206996Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC52000.23616986972052478Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC50260.22826726254141488Illumina Single End PCR Primer 1 (98% over 50bp)
ACATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC45530.20678488785337484Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCTCGGTGGTCGCC40050.18189621696744263Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC37830.17181358022168175Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCC35820.1626847064113307Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGC34320.15587211401554632Illumina Single End PCR Primer 1 (97% over 49bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCC34060.15469126466694372Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC33520.15223873140446134Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC31300.1421560946587005Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCCGTGGTCGCC30980.14070274161426646Illumina Single End PCR Primer 1 (98% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGC30420.13815937378650697Illumina Single End PCR Primer 1 (97% over 49bp)
AGATCGGAAGAGCGTCGTGTTGGGAAAGAGTGTAGATCTCGGTGGTCGCC30210.13720561085109717Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTGGCC29810.13538891954555468Illumina Single End PCR Primer 1 (97% over 46bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC23010.10450516735133221Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)