H222TAFX3_n02_vk13-1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H222TAFX3_n02_vk13-1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740328
Sequences flagged as poor quality	0
Sequence length	76
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	250207	33.7967765639014	Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC	9872	1.333463005586713	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	6122	0.8269307658227164	Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	3782	0.5108546482099826	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC	3509	0.4739791011551637	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC	3202	0.43251099512648444	Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	2737	0.3697009973957489	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC	2669	0.36051587944802843	Illumina Single End PCR Primer 1 (98% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	2577	0.3480889551658184	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC	2371	0.32026345079478286	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC	1995	0.2694751515544461	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCC	1959	0.2646124420527118	Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	1921	0.2594795820231033	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC	1809	0.24435115246215192	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	1790	0.24178472244734767	Illumina Single End PCR Primer 1 (98% over 50bp)
ACATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1630	0.22017268021741715	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCTCGGTGGTCGCC	1371	0.18518818685771712	Illumina Single End PCR Primer 1 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1275	0.17222096151975883	No Hit
AGATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCC	1254	0.16938438097708042	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCC	1219	0.16465674673928313	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC	1173	0.1584432845981781	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC	1123	0.15168952140132483	Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	1082	0.14615143557990512	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTTGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1068	0.1442603818847862	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTGGCC	1060	0.14317977977328966	Illumina Single End PCR Primer 1 (97% over 46bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCCGTGGTCGCC	1030	0.1391275218551777	Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGC	940	0.1269707481008418	Illumina Single End PCR Primer 1 (97% over 49bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGC	869	0.11738040436131013	Illumina Single End PCR Primer 1 (97% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTTGTCGCC	852	0.11508412487438	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content