H222TAFX3_n02_vk13-10.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H222TAFX3_n02_vk13-10.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2361662
Sequences flagged as poor quality	0
Sequence length	76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	1054266	44.6408503841786	Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCC	48335	2.0466518917609715	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	28997	1.2278217628094115	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC	15050	0.6372630799835032	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC	14931	0.6322242556301453	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC	11819	0.5004526473305664	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC	11334	0.4799162623609983	Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	11056	0.4681448911825655	Illumina Single End PCR Primer 1 (100% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	10416	0.4410453316350943	Illumina Single End PCR Primer 1 (98% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	9347	0.39578059857845876	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCC	8919	0.3776577681310873	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC	8767	0.37122162273856296	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	8196	0.34704373445480347	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC	7640	0.3235009920979378	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCTCGGTGGTCGCC	6693	0.28340211258003895	Illumina Single End PCR Primer 1 (98% over 50bp)
ACATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	6021	0.25494757505519416	Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	5952	0.25202590379148243	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCC	5904	0.2499934368254221	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC	5617	0.23784097809085297	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTTGGGAAAGAGTGTAGATCTCGGTGGTCGCC	5391	0.2282714461256522	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTAGATCTCGGTGGTCGCC	5368	0.22729755570441496	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTGGCC	5173	0.2190406586547948	Illumina Single End PCR Primer 1 (97% over 46bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC	4948	0.20951346975138693	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCCGTGGTCGCC	4796	0.20307732435886255	Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGC	4244	0.1797039542491686	Illumina Single End PCR Primer 1 (97% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	3675	0.15561075208899497	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTTGTCGCC	3353	0.1419762861916735	Illumina Single End PCR Primer 1 (98% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGC	3344	0.14159519863553718	Illumina Single End PCR Primer 1 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	3240	0.1371915202090731	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGATCTCGGTGGTCGCC	3033	0.12842650641793787	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTTTAGATCTCGGTGGTCGCC	2525	0.10691623102713257	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTGGCC	2515	0.10649280040920335	Illumina Single End PCR Primer 1 (97% over 46bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTGGCC	2502	0.10594234060589534	Illumina Single End PCR Primer 1 (97% over 46bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCTCGGTGGTCGCC	2418	0.10238552341528973	Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGC	2369	0.10031071338743648	Illumina Single End PCR Primer 1 (97% over 49bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content