FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n01_vk13-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n01_vk13-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201805
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG95908143.558852850275116TruSeq Adapter, Index 9 (100% over 49bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT384671.747066611257582TruSeq Adapter, Index 9 (100% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT332971.512259260016214TruSeq Adapter, Index 9 (100% over 48bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT257221.1682233440291034TruSeq Adapter, Index 9 (100% over 48bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT161910.7353512232009646TruSeq Adapter, Index 9 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC142370.6466058529252137TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG133260.6052307084414833TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATT114310.5191649578414074TruSeq Adapter, Index 9 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATG94890.4309645949573191TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATG70550.32041892901505814TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGGATG56390.2561080567988537TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAAG49780.2260872329747639TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATG40870.18562043414380475TruSeq Adapter, Index 9 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTTTG40420.18357665642506943TruSeq Adapter, Index 9 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG38990.17708198500775502TruSeq Adapter, Index 9 (100% over 49bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA33500.15214789683918423TruSeq Adapter, Index 9 (100% over 47bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTGTG31480.1429736057461946TruSeq Adapter, Index 9 (97% over 49bp)
TTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTA31390.14256485020244755TruSeq Adapter, Index 9 (100% over 47bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG28340.12871257899768598TruSeq Adapter, Index 9 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)