FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n01_vk13-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n01_vk13-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739204
Sequences flagged as poor quality0
Sequence length76
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTG302124.087099095784113TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTG235323.183424332119415TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTT216302.9261205296508135TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTT136241.8430636197856072TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTG117781.5933355338986261TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATG115261.5592448092813351TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTT110791.4987743572816163TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTT96301.3027526907321931TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTG95711.294771132190843TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG84701.1458271329700598TruSeq Adapter, Index 3 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATG80731.092120713632502TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATG77471.0480192206752128TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGGG72870.9857901201833323TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGGTG64020.8660667420630841TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTG57230.7742111785109388TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATT44860.6068690104490777TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATG43250.5850888252769195TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATT43240.5849535446236763TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTT36270.49066292931315303TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATT32820.44399110394424274TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATT32650.44169133283910805TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATG29840.40367746927776366TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGGTT29330.3967781559623595TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGATG28520.3858204230496588TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTG27080.36634000898263536TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTG26730.36160518611912273TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGGTG26300.3557881180296643TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTT24740.3346843361237223TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGATG23280.3149333607502124TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGGG23140.31303943160480735TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGTTG21970.2972115951753508TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGATT20910.2828718459315696TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATT19460.26325615121130297TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATT19080.2581154863880607TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTGTG19020.2573038024686014TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTT18830.2547334700569802TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTG18640.2521631376453591TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGGT18020.24377573714427953TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGTTT17880.24188180799887443TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGATG17650.23877035297428045TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGGTT17230.23308856553806526TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGTTG17130.2317357590056331TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATG15900.2150962386567172TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTTTT15850.2144198353905011TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTGTT15060.20373266378428687TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGTTG14040.1899340371534786TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGGATT13840.18722842408861423TruSeq Adapter, Index 3 (97% over 41bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTT13530.18303472383807448TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTT13370.180870233386183TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGTTT13270.17951742685375077TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTGTT12830.17356507811104918TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTGTG12140.1642307130372671TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTTTG12090.163554309771051TruSeq Adapter, Index 3 (97% over 41bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTT12070.1632837484645646TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGTTT12030.16274262585159172TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATT11970.1619309419321324TruSeq Adapter, Index 3 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGATT11450.15489634796348506TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGAAG11270.15246129620510712TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTTTG10880.1471853507286216TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGTG10790.14596782484943263TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTTTT10770.1456972635429462TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGCGTATG10730.14515614092997334TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTGTG10420.14096244067943356TruSeq Adapter, Index 3 (97% over 46bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTT9670.13081639168619216TruSeq Adapter, Index 3 (97% over 42bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTT9590.12973414646024642TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGAG9190.1243229203305177TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGGTG9020.12202314922538297TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGTATG8650.1170177650553839TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTGGGTTT8600.1163413617891678TruSeq Adapter, Index 3 (97% over 43bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTT8420.11390631003078988TruSeq Adapter, Index 3 (97% over 42bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTT8100.10957732912700688TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGTT8030.10863036455430436TruSeq Adapter, Index 3 (97% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGG7510.10159577058565701TruSeq Adapter, Index 3 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)