FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n01_vk13-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n01_vk13-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662041
Sequences flagged as poor quality0
Sequence length76
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTG6689710.104661191678462TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTG232213.5074866964432716TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTT221833.3506988237888593TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATG197092.977005955824488TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATG164952.4915375331739273TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATG135692.0495709480228568TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTT118781.7941487007602248TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATG108661.641288077324516TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG98721.4911463187325256TruSeq Adapter, Index 2 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGGG87411.3203109777189026TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTGTG87301.318649449203297TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGGTG78421.184518783579869TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTTTG76451.1547623183458426TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATT63270.9556809925669256TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGTTG59760.902663128114422TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATG54900.8292537773340322TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTTTT43930.6635540699140989TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATG42780.6461835445236775TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTGTT41480.6265472984301577TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATT39590.5979992175711172TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGGATG38310.5786650675713437TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTTTG37430.5653728394464995TruSeq Adapter, Index 2 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATT37250.5626539746027814TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATT34790.5254961550719669TruSeq Adapter, Index 2 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATT29500.4455917382760282TruSeq Adapter, Index 2 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGGGTG26620.402089900776538TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGGTTG26620.402089900776538TruSeq Adapter, Index 2 (97% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTT24600.37157819530814556TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGTTT24140.364629985151977TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTTTG23580.35617129452707613TruSeq Adapter, Index 2 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGGTT22980.3471084117146823TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTG21760.32868054999614826TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTGTG20560.3105547843713607TruSeq Adapter, Index 2 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATT20460.3090443039026284TruSeq Adapter, Index 2 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTTTT18670.28200670351232027TruSeq Adapter, Index 2 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATT18010.27203753241868706TruSeq Adapter, Index 2 (97% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTT17990.2717354363249406TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTTTT17380.2625215054656736TruSeq Adapter, Index 2 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGGTTT16960.2561774874969979TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGTTG16880.2549691031220121TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGGATT15750.2379006738253371TruSeq Adapter, Index 2 (97% over 45bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTT15510.23427552070037957TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGGGTT13870.20950364101316987TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGGTG13460.20331067109136744TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTGTG13010.1965135089820721TruSeq Adapter, Index 2 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGGT12630.1907736832008894TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTT11520.17400734999796086TruSeq Adapter, Index 2 (97% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTG10740.16222560234184893TruSeq Adapter, Index 2 (97% over 42bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTTT10740.16222560234184893TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGAG10660.16101721796686308TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGAAG10470.1581473050762717TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGTTT8970.13549009804528722TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTGG8780.13262018515469584TruSeq Adapter, Index 2 (97% over 42bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGG8680.13110970468596356TruSeq Adapter, Index 2 (97% over 42bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTT8620.1302034164047242TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTGTT8360.1262761671860202TruSeq Adapter, Index 2 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTGTT8290.12521883085790758TruSeq Adapter, Index 2 (97% over 46bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTTT8160.1232552062485556TruSeq Adapter, Index 2 (97% over 42bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTG8060.1217447257798233TruSeq Adapter, Index 2 (97% over 42bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTG7460.11268184296742952TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTGTG7280.10996297812371138TruSeq Adapter, Index 2 (97% over 49bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGG7200.10875459374872552TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGAAG6730.10165533554568372TruSeq Adapter, Index 2 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)