FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n01_vk13-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n01_vk13-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740328
Sequences flagged as poor quality0
Sequence length76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG30485541.17836958753418TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATT95421.2888881684874813TruSeq Adapter, Index 1 (97% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT94451.275785867885586TruSeq Adapter, Index 1 (100% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT79841.0784409072735328TruSeq Adapter, Index 1 (100% over 48bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT70770.9559276428826141TruSeq Adapter, Index 1 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTG52410.7079294582941615TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATG47930.6474157400503561TruSeq Adapter, Index 1 (97% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT40230.5434077868188154TruSeq Adapter, Index 1 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATG38570.5209852930052625TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG34150.46128202634507953TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTGTG32840.44358716676932386TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAAG30690.4145459850228547TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATG25830.34889940674944075TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATGTCGTATG24440.3301239450621887TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC23600.3187776228914751TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATG22930.3097275802076917TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTT14130.19086134794307388TruSeq Adapter, Index 1 (97% over 48bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC11900.16073956408510823Illumina Single End PCR Primer 1 (96% over 30bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT11630.15709253195880746Illumina Single End PCR Primer 1 (96% over 29bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG10930.14763726348321285TruSeq Adapter, Index 1 (100% over 49bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGA9860.1331842102419468Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTG9070.12251326439091863Illumina Single End PCR Primer 1 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTG8860.11967668384824023TruSeq Adapter, Index 1 (97% over 45bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTA8740.11805578068099547TruSeq Adapter, Index 1 (100% over 47bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC8550.1154893506661912Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGT8500.11481397434650586Illumina Single End PCR Primer 1 (96% over 31bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTC8370.11305799591532402Illumina Single End PCR Primer 1 (96% over 30bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG8290.1119773938038275TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTG7830.10576393166272247TruSeq Adapter, Index 1 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)