FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n01_vk13-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n01_vk13-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178045
Sequences flagged as poor quality0
Sequence length76
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGGG1683267.728306807251458TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA1393546.398123087447688TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGTG978174.49104586911657TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTA604872.777123521323021TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTG468332.15023105583218TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGTG468122.14926688842517TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTG465582.137605054073722TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGGT427091.9608869421889816TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGTA425141.9519339591238933TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGTT386111.772736559621128TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGTA349411.6042368270628018TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGG329251.51167675598989TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGTT259121.189690754782385TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTT233641.072705109398566TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGA219611.0082895440635984TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGGG207850.9542961692710664TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGGA194790.8943341391018091TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTT179400.8236744419881132TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGA156860.7201871403024271TruSeq Adapter, Index 14 (97% over 44bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGG117190.5380513258449664TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGG111030.5097690819060212TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGT105000.48208370350474855TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGGA98810.45366372136480193TruSeq Adapter, Index 14 (97% over 44bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGG91320.41927508384812984TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGGT90890.4173008363004438TruSeq Adapter, Index 14 (97% over 44bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGG67800.3112883342630662TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGT53310.24476078317941088TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGGC51580.23681788025499934TruSeq Adapter, Index 14 (97% over 45bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGG42100.1932926087385706TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGTC41530.1906755829195448TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTC38560.1770395010204105TruSeq Adapter, Index 14 (97% over 45bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT36430.16726008874931417Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC34810.1598222258952409Illumina Single End PCR Primer 1 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGC31690.14549745299109984TruSeq Adapter, Index 14 (97% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGG31640.14526788932276422TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAGCGGGGG31420.1442578091820876TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGGTT29740.13654446992601163TruSeq Adapter, Index 14 (97% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGT29650.13613125532300757TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTAGTA29590.13585577892100484TruSeq Adapter, Index 14 (97% over 44bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGG29280.13443248417732417TruSeq Adapter, Index 14 (97% over 45bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTC28680.13167772015729703Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGA27270.12520402471023326Illumina Single End PCR Primer 1 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTC25790.11840894012749967TruSeq Adapter, Index 14 (97% over 44bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGG25620.11762842365515863TruSeq Adapter, Index 14 (97% over 45bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTG25530.11721520905215456Illumina Single End PCR Primer 1 (96% over 28bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGG25530.11721520905215456TruSeq Adapter, Index 14 (97% over 44bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGT25450.11684790718281762TruSeq Adapter, Index 14 (97% over 44bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGT25220.11579191430847388Illumina Single End PCR Primer 1 (96% over 31bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGT23060.10587476383637622TruSeq Adapter, Index 14 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGTC23010.1056452001680406TruSeq Adapter, Index 14 (97% over 44bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGT22260.10220174514300667TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTCTCGGGTG22250.10215583240933956TruSeq Adapter, Index 14 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)