FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n01_vk13-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n01_vk13-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942035
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTA101751252.394112361517685TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTG714963.6814990461037005TruSeq Adapter, Index 13 (97% over 39bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGT285171.4684081388852415TruSeq Adapter, Index 13 (97% over 39bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGT222341.144881528911683TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTT168330.8667711961936834TruSeq Adapter, Index 13 (97% over 39bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGT168150.8658443333925495TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTAT137520.7081231800662706TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATGTCGTA106580.548805763026928TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATACTCTCGTA105580.5436565252428509TruSeq Adapter, Index 13 (97% over 39bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGT104110.5360871457002577TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATATCGTA81580.42007481842500266TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCGCGTA80180.4128658855272948TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAAGCTCGTA61950.3189952807235709TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTC49600.2554021940902198TruSeq Adapter, Index 13 (97% over 39bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTA35300.18176809377791853TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTAGTA35060.18053227670974004TruSeq Adapter, Index 13 (97% over 39bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCGTA34340.1768248255052046TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCTCCTA31700.16323083775524128TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATCGCGTG31550.16245845208762974TruSeq Adapter, Index 13 (97% over 39bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT22910.11796903763320434Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC20970.10797951633209493Illumina Single End PCR Primer 1 (96% over 30bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATAATGTCGT20290.10447803463892258TruSeq Adapter, Index 13 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAATACTCTCGTG20110.10355117183778871TruSeq Adapter, Index 13 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)