FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n01_vk13-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n01_vk13-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659214
Sequences flagged as poor quality0
Sequence length76
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG946965.707280676272018TruSeq Adapter, Index 12 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG676184.075303125455789TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGGTG451622.7218912087289526TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGGTG391152.357441535570457TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATG230461.3889709223764988TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGGTT220661.3299068112973975TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTTG210941.2713248562271051TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATT204381.231788063504768TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATT199231.2007492704376892TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATG199201.200568461934386TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATG199181.2004479229321836TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGATG194071.1696502078695092TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGTTG190971.150966662528161TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGGATG190951.1508461235259588TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGGTT172651.040552936510902TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGATG154500.9311637920123624TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGTTT128490.7744028196483396TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGTTG122910.7407724380339125TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTTTG122370.7375178849744517TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTG122090.7358303389436203TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTTT121910.7347454879238001TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGGTTG118820.7161222120835529TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGTTT117570.7085885244459124TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGGGTG110590.666520412677328TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTGTG110520.6660985261696201TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGGTG100940.6083603441147435TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTGTG92630.5582763886997096TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATT79830.48113142729027114TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGGTTT78290.4718499241206981TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTTTT77360.4662448605182936TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGGATT77170.4650997399973722TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGGGG76260.45961521539717TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGATT72150.4348444504446081TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTT64650.38964232461876525TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATT59470.35842272304838313TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGGGTT59340.3576392195340685TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGTTG57270.34516343280613593TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTGTT57150.3444401987929224TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGTGTG56440.3401610642147427TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATT56290.3392570216982258TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGGTG55890.33684624165418087TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGGGTG51790.31213574620272005TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGTTG51380.309664696657574TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTGTT50660.3053252925782931TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTTTG45810.27609458454424807TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTGTG44420.26771712389119184TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGATT43610.26283529430200087TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGGGGGTG42270.25475918115445023TruSeq Adapter, Index 12 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGATG40170.24210258592321424TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGTGTT37300.2248052391071917TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGGTTG36530.2201644875224052TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGTTTG36300.21877828899707932TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGGTTT36180.21805505498386585TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTGTG35670.21498131042770852TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGGGTT34750.20943651632640514TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGTTTT34360.20708600578346134TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGGTT34050.2052176512493265TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTTTT33160.19985366565132648TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTTTG30640.18466575137384328TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGTTT28790.17351589367013537TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTATG28770.17339535466793313TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAAG28540.17200915614260728TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGTTT27870.167971099568832TruSeq Adapter, Index 12 (97% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGTT27630.16652463154240502TruSeq Adapter, Index 12 (97% over 41bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT25610.154350192319978Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGGGG25070.15109563926051733TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTTTT25010.15073402225391058TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGTATG24730.14904647622307912TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGTATG24180.1457316536625173TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTGTG23900.14404410763168585TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGATT23710.14289898711076449TruSeq Adapter, Index 12 (97% over 41bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC23690.14277844810856224Illumina Single End PCR Primer 1 (96% over 30bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTT23200.13982524255460718TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGGATG22510.13566664697862962TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTATT22300.13440098745550605TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGGTT21920.13211074641366333TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTGTT21540.12982050537182063TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTGTT19260.11607905912076441TruSeq Adapter, Index 12 (97% over 43bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGA19050.11481339959764081Illumina Single End PCR Primer 1 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGGGGATG18820.11342720107231495TruSeq Adapter, Index 12 (97% over 42bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTG18310.11035345651615766Illumina Single End PCR Primer 1 (96% over 28bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGTT18290.1102329175139554TruSeq Adapter, Index 12 (97% over 41bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTC17920.10800294597321382Illumina Single End PCR Primer 1 (96% over 30bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT17740.10691809495339359TruSeq Adapter, Index 12 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGAAG17460.10523054892256213TruSeq Adapter, Index 12 (97% over 47bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGGGGGTT17330.10444704540824752TruSeq Adapter, Index 12 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGTATT16720.10077060584107896TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGGGGTTG16620.10016791083006774TruSeq Adapter, Index 12 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)