FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n01_vk13-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n01_vk13-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590997
Sequences flagged as poor quality0
Sequence length76
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG65053940.88876346089905TruSeq Adapter, Index 11 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATT527483.315405371600324TruSeq Adapter, Index 11 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTTG422942.6583331081076835TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTGTG334182.1004439354693942TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATG306861.9287277097316966TruSeq Adapter, Index 11 (97% over 49bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT216901.3632960967242553TruSeq Adapter, Index 11 (100% over 48bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT164921.0365827214004804TruSeq Adapter, Index 11 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTTT147100.9245774819185706TruSeq Adapter, Index 11 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGGATG135270.8502215906126787TruSeq Adapter, Index 11 (97% over 49bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT119250.7495300116844972TruSeq Adapter, Index 11 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAAG113100.7108750047925924TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGTCGTATG101940.6407303093594772TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTGTG95480.6001268387055413TruSeq Adapter, Index 11 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTGTT74390.46756844921769186TruSeq Adapter, Index 11 (97% over 48bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAT71500.44940373866198363TruSeq Adapter, Index 11 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTTTG69620.4375872487503119TruSeq Adapter, Index 11 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGGGTG54750.34412384184256795TruSeq Adapter, Index 11 (97% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTT53410.33570145009701463TruSeq Adapter, Index 11 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATT51030.32074227669819616TruSeq Adapter, Index 11 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGGATG46840.2944065890759065TruSeq Adapter, Index 11 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGGGTG44420.27919600099811626TruSeq Adapter, Index 11 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC39600.2489005321820217TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGGTTG37060.23293570006731626TruSeq Adapter, Index 11 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGTCGTTTG36770.23111294364477117TruSeq Adapter, Index 11 (97% over 46bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTT34290.21552523354852338TruSeq Adapter, Index 11 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGTCGTATT32020.20125745051687716TruSeq Adapter, Index 11 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAAT30980.19472066886361195TruSeq Adapter, Index 11 (97% over 47bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTTTT28240.17749876335404782TruSeq Adapter, Index 11 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCCTATG27130.17052200601258205TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGGATT26800.1684478349110652TruSeq Adapter, Index 11 (97% over 48bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG26310.1653680050936614TruSeq Adapter, Index 11 (100% over 49bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTT26020.16354524867111628TruSeq Adapter, Index 11 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAGCTCGTATG25450.15996258949576903TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTATG25020.1572598816968228TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGTCGTGTG22830.14349492802312008TruSeq Adapter, Index 11 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGTCGTTTT22790.14324351334414834TruSeq Adapter, Index 11 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGGTTG22440.14104363490314564TruSeq Adapter, Index 11 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACCTCTCGTATG21080.13249553581810652TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTGTT20440.12847290095455868TruSeq Adapter, Index 11 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGGCGTATG18700.11753636241928804TruSeq Adapter, Index 11 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTAG18320.11514792296905652TruSeq Adapter, Index 11 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCGTATG17720.11137670278448042TruSeq Adapter, Index 11 (97% over 49bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT16720.10509133581018694Illumina Single End PCR Primer 1 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGGTTT16030.10075443259792444TruSeq Adapter, Index 11 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)