FastQCFastQC Report
Fri 4 Jun 2021
H222TAFX3_n01_vk13-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH222TAFX3_n01_vk13-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361662
Sequences flagged as poor quality0
Sequence length76
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTG43961618.614687453157988TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTT1335485.654831216321387TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTG1328605.625699189807856TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG949984.0225061842041745TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGG803743.403281248544457TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTT605592.564253479117672TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATG450831.9089522548103834TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTGTG323381.3692899322595697TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATG306311.2970103257790488TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGTG297381.2591979715979678TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATT292131.236967864156683TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTG265101.1225145681304098TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG216360.9161344849516994TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGTTG198050.8386043388088559TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTTT161450.6836287326467547TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTGTT153730.6509398889426176TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATT130030.5505868324933881TruSeq Adapter, Index 10 (97% over 42bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTT128760.5452092636456869TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATT116630.49384712969087025TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGGTG114210.4836001087369827TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGATG109340.46297903764382875TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGT107330.4544680822234511TruSeq Adapter, Index 10 (97% over 42bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTT97900.41453857495272395TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG95650.40501138604931614TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATG95570.40467264155497273TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGTTG89730.3799442934679052TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGTT84350.3571637262233122TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGTTT79800.3378976331075319TruSeq Adapter, Index 10 (97% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTG77060.3262956341762708TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATG76850.3254064298786194TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTTTG75960.32163789737904913TruSeq Adapter, Index 10 (97% over 46bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGG71450.3025411765104405TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATT71080.30097448322410236TruSeq Adapter, Index 10 (97% over 48bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTT67920.2875940756975384TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGAG64390.27264697488463635TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGTTT60380.25566740710567387TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTGG59430.2516448162353461TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGGTT59140.2504168674433513TruSeq Adapter, Index 10 (97% over 45bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTG56810.24055093404560007TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTGTG56220.23805269339981758TruSeq Adapter, Index 10 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTTTG54540.23093905901860637TruSeq Adapter, Index 10 (97% over 46bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGG53730.2275092710133796TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGATT52700.22314793564870838TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTTTT46590.197276324893232TruSeq Adapter, Index 10 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATT45880.19426996750593437TruSeq Adapter, Index 10 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTTTT45200.19139063930401556TruSeq Adapter, Index 10 (97% over 46bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTT44850.18990863214126324TruSeq Adapter, Index 10 (97% over 42bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTG44170.18702930393934442TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATT43090.18245625326570863TruSeq Adapter, Index 10 (97% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGT42760.18105893222654215TruSeq Adapter, Index 10 (97% over 42bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGG41500.17572370644063376TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATT39550.16746680939101363TruSeq Adapter, Index 10 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGTTG38780.1642063936329585TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTGTG37330.15806664967298453TruSeq Adapter, Index 10 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGGTG37260.15777024824043406TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGAAG37010.15671167169561095TruSeq Adapter, Index 10 (97% over 45bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTT35430.150021467932329TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGAAG30490.12910399540662465TruSeq Adapter, Index 10 (97% over 42bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGT29170.12351471124995872TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTG28290.11978852181218141TruSeq Adapter, Index 10 (97% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTG27840.11788308403149984TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTAG27800.11771371178432816TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGAT27570.1167398213630909TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGAG27220.11525781420033858TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTGTT25560.10822886594271323TruSeq Adapter, Index 10 (97% over 46bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGG25530.10810183675733445TruSeq Adapter, Index 10 (97% over 42bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT25520.10805949369554152Illumina Single End PCR Primer 1 (96% over 29bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTTT25280.10704326021251136TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTGT24620.10424861813417838TruSeq Adapter, Index 10 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)