FastQCFastQC Report
Wed 29 Jun 2022
temp_l01_n02_LC_CD021_Post_SC_Collection_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametemp_l01_n02_LC_CD021_Post_SC_Collection_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50073
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAA2508550.09685858646377No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG34066.802068979290237No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG31766.342739600183732No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG31726.334751263155793No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA28555.701675553691611No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG20914.175903181355221No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG12672.5303057535997446No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT9821.9611367403590758No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG9071.8113554210852156No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG7371.4718510973977992No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAG4000.7988337027939209No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA2590.5172448225590638No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGTCCAGAA2500.49927106424620055No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAA2380.4753060531623829No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGACCAGAG1880.3754518403131428No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG1820.363469334771234No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1700.3395043236874164No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA910.181734667385617No Hit
CCTGTTCGCCACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT890.1777404988716474No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG880.17574341461466259No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTGCAGTCCAGTG840.1677550775867234No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG840.1677550775867234No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG820.16376090907275379No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG790.15776965630179937No Hit
CCTGTTTGCTCCCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG750.14978131927386018No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG660.13180756096099694No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTTCGTCCAGAA560.11183671839114892No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG530.1058454656201945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)