FastQCFastQC Report
Wed 29 Jun 2022
temp_l01_n02_LC_CD002_EOS_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametemp_l01_n02_LC_CD002_EOS_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41026
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG840520.487008238677912No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG833020.30419733827329No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG728817.764344561985084No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA40879.96197533271584No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT28917.046750840930143No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG25706.264320187198362No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG15033.663530444108614No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2760.672744113489007No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGAG2300.5606200945741725No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1540.3753717154974894No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGGT1360.33149709940038025No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTGCAGACCAGAG1350.3290596207283186No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1330.3241846633841954No Hit
CCTGTTCGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG1210.294934919319456No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTGACGGCCCAGAG1140.27787256861502463No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAG1050.25593526056647004No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG1030.2510603032223468No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG920.22424803782966898No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTGACGGCCCAGAG830.20231072978111445No Hit
CCTGTTCGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG820.1998732511090528No Hit
CCTGTTCGCTACCCATGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG780.1901233364208063No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTACAGACCAGAG710.173060985716375No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG700.17062350704431337No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG700.17062350704431337No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG690.16818602837225174No Hit
CCTGTTCGCTCCCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG670.1633110710281285No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG630.15356115633988204No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG590.14381124165163556No Hit
CCTGTTCGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTGCAGACCAGAG580.1413737629795739No Hit
CCTGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG550.13406132696338907No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAA550.13406132696338907No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG530.12918636961926583No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTTTCGTCCAGAA510.1243114122751426No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG500.12187393360308096No Hit
CCTGTTTGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT470.11456149758689613No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTGCAGACCAGGT440.10724906157071126No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG440.10724906157071126No Hit
CCCGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG430.10481158289864963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)