FastQCFastQC Report
Wed 29 Jun 2022
temp_l01_n01_LC_CD027_Post_SC_Collection_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametemp_l01_n01_LC_CD027_Post_SC_Collection_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49824
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1323026.553468208092486No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA1055421.18256262042389No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA841916.89747912652537No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA506510.165783558124598No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA16463.3036287732819525No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA13902.789820166987797No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA13472.7035163776493256No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA13282.6653821451509314No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA9561.9187540141297366No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA8571.720054592164419No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA2760.5539499036608864No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2460.49373795761079No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA2370.47567437379576105No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA1810.36327874116891457No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1250.2508831085420681No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA1200.24084778420038533No Hit
TACGTATGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA1160.23281952472703915No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA1130.22679833012202955No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1050.2107418111753372No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA660.13246628131021193No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA630.12644508670520233No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA600.12042389210019266No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTA520.10436737315350034No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGTCTA510.10236030828516378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)