FastQCFastQC Report
Wed 29 Jun 2022
temp_l01_n01_LC_CD004_EOS_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametemp_l01_n01_LC_CD004_EOS_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40140
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA650016.19332336821126No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA528213.158943697060288No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA438910.93423019431988No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGCA35098.741903338315895No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA28076.993024414549079No Hit
TACGTAGGCGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA25686.3976083707025415No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA22785.6751370204285No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA18154.521674140508222No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA17684.404583956153463No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA13003.2386646736422517No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA12673.156452416542103No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA11112.767812655705032No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4441.1061285500747384No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCACGTA2430.6053811659192826No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGTA2080.5181863477827603No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1980.49327354260089684No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTA1890.47085201793721976No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1250.31141006477329347No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGCA1030.25660189337319383No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1010.25161933233682116No Hit
TACGTAGGCGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1010.25161933233682116No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA950.23667164922770303No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA900.2242152466367713No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGCGCGCGCA820.2042850024912805No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGGGCTCGTA810.20179372197309417No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA640.15944195316392626No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGCGCGCGTA610.1519681116093672No Hit
TACGTAGGCGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA570.14200298953662183No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA530.13203786746387644No Hit
TACGTAGGCGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA520.12954658694569007No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGCGTA520.12954658694569007No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCATGTA510.12705530642750373No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA480.1195814648729447No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA470.11709018435475833No Hit
GGATGAGCCCCTCGCCGGCCAGGGCTTCGGTGCCCGCGCCAACGGTCATG460.114598903836572No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA450.11210762331838565No Hit
TACGGAGGGTGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGAGTTCGTA440.1096163428001993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)