FastQCFastQC Report
Wed 29 Jun 2022
temp_l01_n01_LC_CD003_Activation_Visist_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametemp_l01_n01_LC_CD003_Activation_Visist_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47341
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1340628.317948501299085No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1086922.958957351978203No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA40328.516930356350732No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA27575.823704611224943No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA27565.821592277307197No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA23574.978771044126655No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA21674.577427599754969No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA11592.4481950106672863No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA10752.2707589615766457No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA6351.3413320377685305No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA5201.098413637227773No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAACGCA5061.068840962379333No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA4150.8766185758644727No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3990.8428212331805413No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA2230.47105046365729497No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1770.37388310344099196No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1280.2703787414714518No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGTGCA1050.22179506136330032No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1010.2133457256923174No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1010.2133457256923174No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTCGTA890.18799771867936882No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA870.1837730508438774No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGAGTGCA790.16687437950191167No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA710.14997570815994593No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCACGCA690.1457510403244545No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGGGCACGCA510.10772902980503159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)