FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636028
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC32410.5095687611237241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTC554.39195E-879.09067
TACGGGC554.059997E-665.908845
TAGGTAG1700.063.970346
ACGGTCT808.079951E-963.437266
TCGTACG606.8029076E-660.416442
TAGTACG1200.060.416442
GGTCTCG753.7591053E-757.9997838
AGGTAGT2000.054.3747947
ATACGGT553.2181706E-452.727074
ACGGTCC553.2181706E-452.727076
CGCCCTA701.6953194E-551.785522
CTAGTAC2650.049.2450941
CCTAGGT2250.048.333154
CGGTCGC450.0096376848.333157
AGTACGG1351.9099389E-1048.333153
CTCGTAC1109.951873E-846.1361851
GTCTCGT1109.951873E-846.1361859
ACGGGCT700.001056370441.4284136
CTAGGTA2650.041.037585
CGGTCCC750.001483007738.666527
Produced by FastQC (version 0.11.5)