FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n02_45_75_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n02_45_75_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119420
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC40080.35804255775312216No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT38460.3435707777241786No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG35990.321505779778814No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG33290.2973861463972414No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG32860.29354487145128727No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC32110.28684497328973935No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC31400.2805024030301406No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA31390.2804130710546533No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC29210.2609387003984206No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT28040.2504868592664058No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG27210.2430723053009594No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA26320.2351217594825892No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG26250.23449643565417808No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG25140.22458058637508713No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG25090.22413392649765057No Hit
GTGTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTA24770.2212753032820568No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC24120.2154687248753819No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT23960.21403941326758497No Hit
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC23200.20725018313054977No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC22400.20010362509156526No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA21970.1962623501456111No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG21640.19331439495453004No Hit
ACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGT21250.18983044791052509No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT21080.1883118043272409No Hit
CTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACA20740.1852745171606725No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG20500.18313054974897713No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC20470.18286255382251523No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT20260.1809865823372818No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA19960.17830662307266262No Hit
GTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATCAT19840.17723463936681497No Hit
GTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAA19630.17535866788158153No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT19590.17500133997963233No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT19540.17455468010219577No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG19350.17285737256793696No Hit
CTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT19110.17071340515624164No Hit
GCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTG19040.1700880813278305No Hit
ATCATAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGC18250.16303085526433334No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA18050.1612442157545872No Hit
GCATAACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGC17850.15945757624484108No Hit
AATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAA17510.15642028907827268No Hit
TAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGCCCCT17470.15606296117632346No Hit
GTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTA16990.15177502635293277No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG16800.15007771881867396No Hit
GTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGT16610.14838041128441515No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC16520.1475764235050294No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG16520.1475764235050294No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG16250.14516446016687212No Hit
GTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCACATGT16240.14507512819138482No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC15710.1403405334905576No Hit
AGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATA15620.13953654571117186No Hit
GCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCT15170.13551660681424307No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT15030.1342659591574208No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC15030.1342659591574208No Hit
TACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCA14950.13355130335352236No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC14940.13346197137803506No Hit
GTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAAT14920.13328330742706046No Hit
CTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTT14870.1328366475496239No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA14870.1328366475496239No Hit
GCATACTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAA14790.13212199174572548No Hit
GTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAAC14760.13185399581926355No Hit
CACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTA14680.1311393400153651No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT14640.1307820121134159No Hit
GTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTG14500.1295313644565936No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT14420.12881670865269515No Hit
GTTGTACCTCGGTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGT14370.12837004877525862No Hit
CAATAATAGCTCATACCTCCTAAGTAAAGTTGAGTCACATCTGTGACATC14350.12819138482428402No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA14340.1281020528487967No Hit
ACATAAGAATGGTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTG14340.1281020528487967No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT14230.12711940111843634No Hit
ACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCT14180.1266727412409998No Hit
TTCTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTA14060.12560075753515212No Hit
ATGGAAAACTAATGGGTGGTTTATGTGATTTACAATAATAGCTCATACCT14000.1250647656822283No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT13910.12426077790284255No Hit
GTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATC13660.1220274785156599No Hit
GAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACA13440.12006217505493917No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA13410.11979417912847724No Hit
ACCTGGAGCATTGCAAACATACGGATTAACAGACAAGACTAATTTATGTG13210.11800753961873113No Hit
GCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTATTTGTACTTT13100.11702488788837076No Hit
GTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAG13060.11666755998642155No Hit
GCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTAT13050.11657822801093423No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG13030.11639956405995963No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG12750.11389826874631506No Hit
TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT12750.11389826874631506No Hit
CTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTAGG12720.11363027281985315No Hit
GGTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAG12700.11345160886887852No Hit
GGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAG12660.1130942809669293No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG12660.1130942809669293No Hit
ACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTAT12610.11264762108949278No Hit
TGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCAT12560.11220096121205624No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC12540.11202229726108162No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA12540.11202229726108162No Hit
ATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTC12500.11166496935913241No Hit
GTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCA12500.11166496935913241No Hit
GGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTAC12330.11014632577584821No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA12250.10943166997194975No Hit
GTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCT12220.10916367404548784No Hit
CTTCACGTACAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTA12040.10755569848671634No Hit
AATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGAT12010.10728770256025442No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG12000.10719837058476712No Hit
TTTCAGTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCA11950.10675171070733058No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA11930.10657304675635597No Hit
CATTTAATTTGTAAGTTGTTGTACCTCGGTAAACAACAGCATCACCATAG11900.10630505082989405No Hit
CTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATT11890.10621571885440675No Hit
AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC11810.1055010630505083No Hit
GTTTTTAGTTACACGATAACCAGTAAAGACATAATTTCGGTTAAGTGGTG11770.10514373514855907No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA11760.10505440317307177No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC11700.10451841132014794No Hit
ACGTATGCAAGCACCACATCTTAATGAAGTCTGTGAATTGCAAAGAACAC11510.10282110378588911No Hit
ATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAA11510.10282110378588911No Hit
GTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTA11500.10273177181040181No Hit
TGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGT11480.1025531078594272No Hit
GTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGAC11480.1025531078594272No Hit
CTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTA11460.1023744439084526No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT11410.10192778403101607No Hit
TGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTAT11400.10183845205552874No Hit
GTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACACGAACCGTTCAATC11400.10183845205552874No Hit
GCATTAAAGTCAGTAACATTATCGCTACCAACACATGTATTTTTATATAA11400.10183845205552874No Hit
GTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAA11390.10174912008004144No Hit
GATTAACAGACAAGACTAATTTATGTGATGTTGATATGACATGGTCGTAA11380.10165978810455414No Hit
CTGTAAGACTGTATGCGGTGTGTACATAGCCTCATAAAACTCAGGTTCCC11370.10157045612906683No Hit
CTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTG11360.10148112415357953No Hit
GAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTCCCTGG11350.10139179217809223No Hit
CTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAA11290.1008558003251684No Hit
GTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGC11280.10076646834968109No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT11280.10076646834968109No Hit
TCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTA11200.10005181254578263No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG11200.10005181254578263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAG501.8189894E-12116.02
GTGATAG1250.0110.21
TCGTGGG203.8661787E-4108.75145
CCCTAGC1950.0104.102571
GAATATT352.7619717E-7103.571431
AGGGTAG800.099.68753
GAGCTGA1750.095.285711
CCTAGCA2100.093.214292
TGATAGA3600.090.6251
AAGGGTA900.088.6111152
CGCCTAA259.387042E-487.01
GAAGGGT950.083.947371
GTCTCAA703.092282E-1182.857141
TAGAGCC4150.080.361444
ATAGAGC4400.077.443183
AGAGCCA4700.070.957455
AGTAGAG7300.069.5205465
GATAGAG5250.063.523812
GTTACTA2750.063.2727249
GAGCCAT5300.062.924536
Produced by FastQC (version 0.11.5)