FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n02_45_75_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n02_45_75_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374614
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG57630.4192449662232452No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT49960.3634474841664642No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA45480.3308565168112648No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC45460.3307110214212863No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC44370.32278152266745425No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG31160.22668181758660977No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG28650.20842214614429944No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT28160.2048575090898245No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC27540.20034715200048886No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC26740.19452733640134612No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC26730.19445458870635685No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA24860.18085076974336067No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG24740.17997779740348926No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC24660.179395815843575No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT24070.1751037018392072No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT23720.17255753251458228No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC22670.16491902454070742No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG22160.16120889209625394No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC22000.1600449289764254No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA21950.15968119050147894No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT21540.1566985350069183No Hit
GTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACC21400.15568006727706832No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT21100.15349763642738978No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT21090.1534248887324005No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC20760.15102421479775413No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT20680.15044223323783984No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC20100.14622286692846137No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT20090.1461501192334721No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC19800.14404043607878284No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC19670.14309471604392215No Hit
GTCTAGTAGTTGCACATGTCAACTTAAAAGGTAAGTTATTCTTTTTAGCA19230.13989381746439364No Hit
GTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCA18830.13698390966482227No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA18370.1336375156953152No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC18070.13145508484563667No Hit
CAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTG17770.12927265399595814No Hit
GTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGT17410.1266537369763439No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA17360.12628999850139747No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT17320.12599900772144035No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG17240.12541702616152606No Hit
GTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGC16920.12308909992186896No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA16700.12148865063210472No Hit
GCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGACATGAC16530.12025193981728688No Hit
ATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATT16480.11988820134234046No Hit
ATTTAAGACATTCAACAACATCTTTAGTTTCTACATCTGAATCAACAAAC16340.1188697336124905No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT16320.11872423822251192No Hit
GTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAA16210.1179240135776298No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT16020.11654180737283339No Hit
CTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGT15950.1160325735079084No Hit
CAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTT15610.11355915187827273No Hit
GTTATAGGTGAGCATATAGTTATTACAACTATCGCCAGTAACTTCTATGT15560.11319541340332631No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC15400.11203145028349777No Hit
CTTCTATGTCAGATTGATGTGACAATTTAAGACATTCAACAACATCTTTA15320.11144946872358351No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC15140.11014001021377637No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA15120.10999451482379781No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG15110.10992176712880852No Hit
ACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTT15090.10977627173882995No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT14850.10803032705908712No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA14770.10744834549917286No Hit
TAATAAAATGATGCAAAGAAGATGTACATTCTAACCATAGCTGAAATCGG14770.10744834549917286No Hit
AACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGT14660.10664812085429073No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG14610.10628438237934432No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC14600.10621163468435503No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA14340.10432019461463363No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT14190.10322897918979437No Hit
CGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAA14070.10235600684992296No Hit
CTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATT13900.10111929603510512No Hit
CACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAA13890.10104654834011584No Hit
CCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTTAAGATTGTCA13870.10090105295013727No Hit
GTAAGAAGACTGGTCAGTAGGATTTATTGGTCTTTTAAACTGTAGTGACA13850.1007555575601587No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA13760.10010082830525516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG100.00709115144.999982
TCCTAGG5900.0115.508473
TTCCTAG6050.0112.644622
TCGTGGG203.866369E-4108.74999145
TAGGTAG6300.0108.17466
CAGGTAT800.099.687494
CTTATCG301.5996737E-596.6666641
AACCCTA353.4377364E-582.857145
TATCGGC353.4377364E-582.857143
GTAGAGC451.2331329E-680.555563
AGGTAGT8950.076.145257
TTATCGG406.664076E-572.499992
ATCGGCA406.664076E-572.499994
AAAGGGT606.8069476E-660.4166685
CTTAACC502.0105102E-458.02
GAGTCAA502.0105102E-458.0145
ATGCGCT1800.056.388896
AGAGCAG651.0938613E-555.7692345
CGCTAGT1750.053.8571439
TATGCGC1900.053.4210475
Produced by FastQC (version 0.11.5)