FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n02_45_25-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n02_45_25-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160024
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA78850.6797273159865658No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC78850.6797273159865658No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG65740.5667124128466308No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC55960.4824038123349172No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC53730.4631800721364386No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC53430.4605939187465087No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG51750.44611145976290145No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT50460.43499100018620307No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT41760.359992551878237No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG40460.3487858871885409No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC37820.3260277373571581No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC34950.30128686992682907No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA34070.2937008199830349No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG32110.2768046178354931No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT31200.2689599525527058No Hit
CAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTT30440.2624083639648835No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA29370.2531844168741337No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG27910.24059847037647494No Hit
CCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTTAAGATTGTCA27480.2368916505175755No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG26350.2271504727488397No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC26150.22542637048888645No Hit
GTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTA25850.22284021709895657No Hit
CCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCT24810.21387488534719973No Hit
ATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAG23930.20628883540340542No Hit
CATCAGCTCCATCCAAATAAGTTGGACCAAACTGTTGTCCATATGTCATT23740.20465093825644984No Hit
GGTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTAT23550.2030130411094943No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC23520.20275442577050132No Hit
GAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAA23520.20275442577050132No Hit
GTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTT23140.19947863147659015No Hit
CTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTAC22780.1963752474086743No Hit
ATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAG22760.19620283718267897No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT22410.19318565822776082No Hit
GCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTG21910.1888754025778777No Hit
ATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTA21260.18327207023302966No Hit
CCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAA20870.17991007082612084No Hit
TATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGG20750.17887560947014888No Hit
ATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCT20380.1756860202892354No Hit
GTCTTTGCCTCCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGT20090.17318607201230318No Hit
GTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACAT19700.16982407260539437No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC18890.16284145845258374No Hit
CTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAAT18590.16025530506265387No Hit
ACTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAAC18170.15663469031675206No Hit
ATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCAATCTTTATAG18120.15620366475176375No Hit
CTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTA17620.15189340910188065No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG17610.151807203988883No Hit
GTGTTACATAGCCAAGTGGCATTGTAACAAGAGTTTCATTTAGATCGTTA17590.15163479376288766No Hit
GTTGTAAACACCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTT16950.1461176665310373No Hit
GTCTTAAGATTGTCAAAGGTGATAACTTCACCATCTAGGTGGAATGTGGT16900.14568664096604897No Hit
CTCTTGTATTTTAATACCCTTATATTTACGCTGTATAGTTGAAACTATGG16850.14525561540106066No Hit
GTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCA16810.14491079494907003No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT16740.1443073591580864No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG16720.14413494893209106No Hit
CTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCTGATAATAAT16630.1433591029151121No Hit
GGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCAT16360.14103156486417523No Hit
CTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCA16270.14025571884719626No Hit
GATTACTAGTGTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATA16180.1394798728302173No Hit
CTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGGCACTTTGAGA16140.13913505237822665No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA16000.1379281807962594No Hit
ATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATA15940.1374109501182734No Hit
ATATAATTGTCTGTTGGCACTTTTCTCAAAGCTTTCGCTAGCATTTCAGT15740.13568684785832016No Hit
ATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAA15720.13551443763232485No Hit
GTATAGGGTCAGCACCAAAAATACCAGCTGATAATAATGGTGCAAGTAGA15690.13525582229333186No Hit
GTTATAAATGGCTTAACTTCCTCTTTAGGAATCTCAGCGATCTTTTGTTC15520.1337903353723716No Hit
CTCTTGAACAACATCACCCACTATATATGGAGCATCTTTCTTTAAGAAAG15350.13232484845141135No Hit
GTGTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTG15320.13206623311241836No Hit
GGTTAATGTTGTCTACTGTTGTAAACACCTTAATAGTCCTCACTTCTCTC15300.13189382288642304No Hit
GTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGG15270.13163520754743005No Hit
CTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAAT15130.13042833596546277No Hit
GACATGTACCTACCCAGAAAACTAGGATCAGTTGTGTGGTAGTACTCAAA15120.13034213085246513No Hit
GCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACA14640.12620428542857734No Hit
ATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGAT14630.1261180803155797No Hit
ATATAAAGTAACAAGTTTTCTGTGAGGAACTTAGTTTCTTCCAGAGTTGT14630.1261180803155797No Hit
CTCTTAAGAAGTTGAATGTCTTCACCTTTGTTAACATTTGGGCCGACAAC14260.12292849113466617No Hit
CTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGAC14210.12249746556967787No Hit
TCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAAATGTTCTTCAGG14170.12215264511768723No Hit
GTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAATTGTCTGT14130.12180782466569658No Hit
GTCCACAACTTGCGTGTGGAGGTTAATGTTGTCTACTGTTGTAAACACCT13990.1206009530837293No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG13940.12016992751874098No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGG13890.11973890195375268No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG13660.11775618435480645No Hit
GTTGTGTGGTAGTACTCAAAAGCCTCAACACGTAGAGTGTCATCATTAGG13640.11758377412881113No Hit
GTTATATAATTGTCTGTTGGCACTTTTCTCAAAGCTTTCGCTAGCATTTC13630.11749756901581346No Hit
ACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTC13550.11680792811183216No Hit
GTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGA13540.11672172299883452No Hit
GCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTA13350.11508382585187893No Hit
TTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGG13100.11292869802693736No Hit
CTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCC12990.11198044178396308No Hit
CTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTT12710.10956669862002855No Hit
AATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTC12690.10939428839403323No Hit
GTTGTTATCTGCCCATTTAATAGAAGTTAAACCATTAACTTGTGGGTATT12670.10922187816803791No Hit
GTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTG12640.10896326282904491No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG12550.10818741681206595No Hit
CAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAG12290.10594608387412674No Hit
TGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTG12280.10585987876112908No Hit
GTCCATATGTCATTGACATGTCCACAACTTGCGTGTGGAGGTTAATGTTG12210.10525644297014546No Hit
ATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGGCA12100.10430818672717117No Hit
GTTAAAACACCCTCTTGAACAACATCACCCACTATATATGGAGCATCTTT11680.10068757198126936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCC151.2300556E-4145.0145
TCACGTT100.007091007145.0145
TTCCTAG8000.0119.6252
TCCTAGG8150.0117.423313
TAGGTAG8400.0112.202386
CAGGTAT950.091.578954
CCTAGGT10450.090.885174
GTAGAGC1250.087.03
AGGTAGT12600.074.801587
ACTGAAC406.663636E-572.53
CGGTATT300.001935820772.5145
AGAGCAG1500.072.55
AGATCTG23150.065.453566
TCGTGGG350.003566647462.142857145
ATCTGTT24550.061.7209788
GACTGTT502.010379E-458.07
GATCTGT27650.056.1121187
GAGCAGG2000.054.3756
GGACAGT953.1399395E-853.4210556
AGGAGTA553.2192172E-452.7272727
Produced by FastQC (version 0.11.5)