FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n02_45_100_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n02_45_100_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508556
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG61220.40581854435632486No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT52770.3498047139118468No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC48540.3217646544112383No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA46570.30870580873364994No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC46430.30777776893930353No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG36070.23910282415767128No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC32950.2184207944550948No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT30510.20224638661077216No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA28110.18633713299340562No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG27950.18527651608558118No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC27430.18182951113515175No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC27300.1809677598975444No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT26910.17838250618472234No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC26790.17758704350385401No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG24630.16326871524822414No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG24430.16194294411344357No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC24000.1590925361736654No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT22990.15239739194302368No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT22920.15193337204585045No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC22870.15160192926215532No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT22640.1500772924571577No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT22140.14676286462020632No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT21300.14119462585412804No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA20240.13416803883979117No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC20170.13370401894261796No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC19990.1325108249213155No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT19730.13078732244610078No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC19670.1303895911056666No Hit
GTCTAGTAGTTGCACATGTCAACTTAAAAGGTAAGTTATTCTTTTTAGCA19450.128931242857408No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC19400.12859980007371286No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA19170.12707516326871526No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC18810.12468877522611027No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA18680.1238270239885029No Hit
CAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTG18180.12051259615155155No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT18110.12004857625437836No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT18050.1196508449139442No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG17830.11819249666568558No Hit
GTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCA17780.11786105388199046No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC17330.11487806882873423No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA17240.114281471818083No Hit
GTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGC16970.11249168078612926No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT16930.11222652655917315No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC16910.11209394944569509No Hit
GTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGT16890.11196137233221704No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC16790.11129848676482677No Hit
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAA16420.10884581016548277No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTAC16410.10877952160874374No Hit
CAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACAC16230.10758632758744123No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT16120.10685715346331194No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA16080.10659199923635583No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT15920.10553138232853139No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT15830.10493478531788014No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA15820.10486849676114111No Hit
ATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATT15550.10307870572918737No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG15480.10261468583201419No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA15440.10234953160505808No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC15410.102150665934841No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG15370.10188551170788489No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA15360.10181922315114586No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG15230.10095747191353852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGA100.0070912167145.06
GTGGGGA100.0070912167145.04
GTTAACG100.0070912167145.01
CGGTATC100.0070912167145.09
GACTGAC100.0070912167145.07
GCTCGGA100.0070912167145.02
CAGGTAT1150.0113.4782644
TCCTAGG6450.0110.155043
TTCCTAG6450.0110.155042
TAGGTAG6650.0106.84216
AGGAGTA900.080.555567
GAAGGAG950.076.315795
AGGTAGT9500.074.7894747
GGAGTAG1000.072.58
TGAAGGA1050.069.0476154
TATGCGC2150.067.441865
TGCGCTA2250.064.444457
CGCTAGT2400.063.43759
ATGCGCT2300.063.043486
GGCGTGT606.8072695E-660.4166688
Produced by FastQC (version 0.11.5)