FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n02_44_50_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n02_44_50_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990132
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC36900.372677582382955No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT36700.3706576496871124No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC34350.3469234405109622No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG34120.3446005179107432No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG31030.31339255775997543No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG30850.31157461833371713No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA29110.29400120387988676No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC28400.2868304428096456No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC28280.28561848319214006No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG26300.2656211495032985No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT25990.26249025382474256No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA25570.25824839516347314No Hit
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC25190.25441052304137224No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG24970.25218859707594543No Hit
GTGTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTA23840.24077597734443487No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG23720.23956401772692934No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG23370.23602913550920482No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC23300.2353221590656599No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG22010.22229359317747532No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC21510.21724376143786886No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA21170.2138098758549365No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT20840.21047698690679628No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC20640.2084570542109537No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT20610.20815406430657732No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT20410.20613413161073474No Hit
CTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACA19680.19876137727090934No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG19620.19815539746215657No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA19580.19775141092298804No Hit
GTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAA19120.19310556572255014No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT19050.19239858927900522No Hit
ACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGT18760.1894696868700335No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG18730.1891666969656571No Hit
GCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTG18420.18603580128710112No Hit
ATCATAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGC17570.17745108732977016No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT17270.1744211882860063No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG17260.17432019165121418No Hit
CTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT17030.17199726905099522No Hit
AATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAA16790.16957334981598413No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA16690.16856338346806285No Hit
GTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTA16630.16795740365931008No Hit
GTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGT16460.1662404608678439No Hit
GTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATCAT16380.16543248778950684No Hit
GCATAACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGC16310.16472551134596194No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG16250.16411953153720918No Hit
TAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGCCCCT16200.16361454836324854No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC16080.16240258874574298No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC15550.15704976710176016No Hit
CACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTA15460.15614079738863101No Hit
CTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTT15430.15583780748425463No Hit
AGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATA15120.15270691180569862No Hit
ACATAAGAATGGTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTG14790.14937402285755838No Hit
GTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCACATGT14760.149071032953182No Hit
GTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAAC14570.14715209689213157No Hit
TTCTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTA14540.14684910698775516No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT14540.14684910698775516No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC14480.1462431271790024No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG14470.14614213054421027No Hit
ACCTGGAGCATTGCAAACATACGGATTAACAGACAAGACTAATTTATGTG14330.14472817765712045No Hit
GCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCT14290.14432419111795194No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG14280.14422319448315982No Hit
ATGGAAAACTAATGGGTGGTTTATGTGATTTACAATAATAGCTCATACCT14220.14361721467440705No Hit
GTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAG14220.14361721467440705No Hit
GCATACTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAA14000.14139528870898022No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT13700.13836538966521636No Hit
GTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTG13590.13725442668250293No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT13500.13634545696937378No Hit
GCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTAT13450.13584047379541314No Hit
GTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATC13440.135739477160621No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC13430.1356384805258289No Hit
GTTGTACCTCGGTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGT13410.13543648725624463No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG13380.13513349735186825No Hit
GTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAAT12950.1307906420558067No Hit
ACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCT12940.13068964542101458No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT12870.12998266897746968No Hit
CAATAATAGCTCATACCTCCTAAGTAAAGTTGAGTCACATCTGTGACATC12830.12957868243830115No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT12820.12947768580350905No Hit
TACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCA12620.12745775310766644No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA12550.12675077666412154No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA12190.12311489781160491No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA12160.12281190790722854No Hit
CTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTAGG12140.12260991463764427No Hit
GTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCA12030.12149895165493087No Hit
GGTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAG11920.12038798867221744No Hit
GGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTAC11840.11958001559388041No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC11810.11927702568950403No Hit
GAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACA11800.11917602905471189No Hit
ATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTC11800.11917602905471189No Hit
ACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTAT11630.11745908626324571No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA11520.11634812328053229No Hit
CCCTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAA11520.11634812328053229No Hit
TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT11520.11634812328053229No Hit
GGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAG11430.11543915356740314No Hit
GTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCT11420.11533815693261101No Hit
GCATTAAAGTCAGTAACATTATCGCTACCAACACATGTATTTTTATATAA11370.11483317375865036No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA11280.1139242040455212No Hit
TTTCAGTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCA11250.11362121414114483No Hit
AATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGAT11220.11331822423676842No Hit
CATTTAATTTGTAAGTTGTTGTACCTCGGTAAACAACAGCATCACCATAG11170.11281324106280778No Hit
CTTCACGTACAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTA11170.11281324106280778No Hit
TGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCAT11120.11230825788884716No Hit
CTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTG11060.11170227808009438No Hit
CTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATT11040.11150028481051011No Hit
GTTTTTAGTTACACGATAACCAGTAAAGACATAATTTCGGTTAAGTGGTG11000.1110962982713416No Hit
TGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGT10990.11099530163654948No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA10990.11099530163654948No Hit
TCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTA10970.11079330836696522No Hit
TCCCAATACCTTGAAGTGTTATCATTAGTAAGCATAACAGAATACATGTC10960.11069231173217307No Hit
GTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTA10920.11028832519300456No Hit
GATTAACAGACAAGACTAATTTATGTGATGTTGATATGACATGGTCGTAA10910.11018732855821244No Hit
TCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACG10910.11018732855821244No Hit
GTATAATGTCTCCTACAACTTCGGTAGTTTTCACATTACACTCAAGAACG10880.10988433865383605No Hit
ATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAA10850.10958134874945967No Hit
TCATCACCCTGTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGG10830.1093793554798754No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT10800.10907636557549903No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT10780.10887437230591476No Hit
GACACGAACCGTTCAATCATAAGTGTACCATCTGTTTTTACGATATCATC10780.10887437230591476No Hit
GCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTATTTGTACTTT10660.10766241268840922No Hit
GTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTGAATTGCAAAGA10650.10756141605361709No Hit
TGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTAT10570.10675344297528006No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC10550.10655144970569581No Hit
GTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGAC10530.10634945643611154No Hit
CTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTT10490.10594546989694303No Hit
GATATGACATGGTCGTAACAGCATTTACAACATAAGAATGGTCTACGTAT10460.10564247999256665No Hit
ATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGT10450.10554148335777452No Hit
CTGTAAGACTGTATGCGGTGTGTACATAGCCTCATAAAACTCAGGTTCCC10420.10523849345339814No Hit
CTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAA10390.10493550354902174No Hit
CAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGC10360.10463251364464536No Hit
CTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAACAG10350.10453151700985322No Hit
CTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCAT10340.10443052037506109No Hit
AGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAA10310.10412753047068471No Hit
GTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATC10280.10382454056630834No Hit
GTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGG10260.10362254729672407No Hit
TGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTT10260.10362254729672407No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG10240.10342055402713982No Hit
GGTCGTAACAGCATTTACAACATAAGAATGGTCTACGTATGCAAGCACCA10230.10331955739234769No Hit
GTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACACGAACCGTTCAATC10170.10271357758359491No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT10160.1026125809488028No Hit
CTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTA10140.10241058767921853No Hit
CTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAA10110.10210759777484214No Hit
GAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTAT10080.10180460787046575No Hit
GAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTCCCTGG10060.1016026146008815No Hit
ACGTATGCAAGCACCACATCTTAATGAAGTCTGTGAATTGCAAAGAACAC9950.10049165161816807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG100.007090851145.02
CGGTTCC100.007090851145.0145
GATCCAC100.007090851145.0145
GAAAGCG100.007090851145.04
TTGACTA100.007090851145.02
GACATCC100.007090851145.0145
AAGGGTA1900.0122.105272
GAAGGGT1900.0122.105271
TCCCTAG301.1017073E-7120.833342
AGGGTAG2050.0113.170733
CGCCTAA203.8660452E-4108.751
GTGATAG1250.0104.400011
TACCCTT607.2759576E-1296.666687
CCCTAGC1200.096.666681
TGATAGA3900.094.807691
GAGCTGA1850.094.054061
TAGAGCC4100.093.719514
CCTAGCA1250.092.82
ATAGAGC4100.091.951223
AGAGCCA4200.091.488095
Produced by FastQC (version 0.11.5)