FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n02_44_50_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n02_44_50_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029175
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG41210.40041781038210217No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT37540.3647581800957077No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA36870.3582481113513251No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC33440.3249204459882916No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC33160.3221998202443705No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG26880.2611800714164258No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT24970.24262151723467826No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC24010.2332936575412345No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG21060.20462992202492286No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT20980.20385260038380254No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA20850.20258945271698206No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC19110.18568270702261522No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT17120.16634683119974736No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC17030.165472344353487No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC16480.16012825807078485No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC16100.15643598027546335No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC16050.15595015424976316No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT16010.155561493429203No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA15820.15371535453154225No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT15790.15342385891612215No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT15440.15002307673622076No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT15160.14730245099229966No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG15040.1461364685306192No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC15030.14603930332547915No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC14990.145650642504919No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA14800.14380450360725824No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC14610.1419583647095975No Hit
GTCTAGTAGTTGCACATGTCAACTTAAAAGGTAAGTTATTCTTTTTAGCA14610.1419583647095975No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT14290.13884907814511624No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT14170.13768309568343576No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG13950.1355454611703549No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT13860.13467097432409453No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC13720.133310661452134No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA13340.1296183836568125No Hit
GTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGT12990.1262176014769111No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG12990.1262176014769111No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG12980.12612043627177108No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG12380.12029052396336873No Hit
CTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAAC12360.12009619355308865No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC12250.11902737629654819No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG12240.11893021109140817No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC12240.11893021109140817No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA12210.11863871547598806No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC12160.11815288945028785No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA11840.1150436028858066No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT11750.11416911603954623No Hit
CTTCTATGTCAGATTGATGTGACAATTTAAGACATTCAACAACATCTTTA11710.11378045521898608No Hit
GACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAAC11710.11378045521898608No Hit
GTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGC11650.11319746398814584No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT11620.11290596837272573No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT11520.11193431632132533No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC11230.1091165253722642No Hit
GTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCA11100.10785337770544369No Hit
GGTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTAT11090.10775621250030364No Hit
GGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAA10980.10668739524376321No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA10970.10659023003862315No Hit
CAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTG10940.10629873442320305No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG10890.10581290839750286No Hit
ATTTAAGACATTCAACAACATCTTTAGTTTCTACATCTGAATCAACAAAC10740.10435543032040226No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA10660.10357810867928194No Hit
CCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCT10510.10212063060218135No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT10440.10144047416620108No Hit
GTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAA10440.10144047416620108No Hit
GTTATAGGTGAGCATATAGTTATTACAACTATCGCCAGTAACTTCTATGT10410.10114897855078096No Hit
GTTACCAGACACAAATGTGTTGTCTGTAGTAATGATTTGTGGTTCATAAA10360.10066315252508078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG100.007090892145.01
CAGGTAT1000.0116.04
TCCTAGG4850.0109.123713
TTCCTAG5050.0106.2376252
TAGGTAG5250.0100.809526
ATAGGCG406.66328E-572.55
GGCGTGT406.66328E-572.58
AGGTAGT7400.071.520267
TATGCGC1900.068.684215
TGCGCTA2100.062.1428577
ATGCGCT2200.059.318186
CGCTAGT2300.056.7391329
GTTCCTA9550.055.418851
CCTAGGT10000.052.9254
GTACCAC553.2190466E-452.7272721
CTAGGTA10450.050.645935
GGGTACT450.00963921748.3333328
ATATGCG2800.046.6071434
GGATTAA951.9209401E-645.7894741
GGTGGAT657.3366833E-444.6153836
Produced by FastQC (version 0.11.5)