FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n02_44_25_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n02_44_25_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189646
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG63710.5355374624047826No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA59710.5019140147573312No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC53130.44660344337727353No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC52710.4430729813742912No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC49870.4192003335446007No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT36740.30883136664184135No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT34210.28756453600482834No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC33810.28420219124008317No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG32510.27327457075466144No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT30040.25251209183236023No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG28750.24166852996605712No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC27240.22897567847914424No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC25440.21384512703779107No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC24720.20779290646124982No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT23200.1950159963552183No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA22370.1880391309683721No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG21990.18484490344186422No Hit
CAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTT20300.17063899681081598No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG20300.17063899681081598No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG20210.16988246923874833No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT18810.15811426256214034No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA18520.1556765626077001No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG18220.15315480403414125No Hit
CCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCT17830.14987651788851475No Hit
GGTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTAT17770.14937216617380294No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC17530.14735475931495587No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG17370.1460098214090578No Hit
CCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTTAAGATTGTCA17190.1444967662649225No Hit
GTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTA16590.1394532491178048No Hit
CATCAGCTCCATCCAAATAAGTTGGACCAAACTGTTGTCCATATGTCATT16570.13928513187956754No Hit
ATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAG16260.13667931468689004No Hit
ATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAG16240.13651119744865278No Hit
GCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTG16130.13558655263834787No Hit
GTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACC16110.13541843540011061No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT16110.13541843540011061No Hit
GAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAA16030.13474596644716158No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC15780.13264450096919586No Hit
ATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTA15550.13071115272946743No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA15410.12953433206180662No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT15320.12877780448973897No Hit
GTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTT15150.12734880796472228No Hit
GTCTTTGCCTCCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGT15050.126508221773536No Hit
TATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGG14590.12264152529407907No Hit
CTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTAC14410.12112847014994377No Hit
CCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAA14270.11995164948228297No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT13780.11583277714547016No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG13750.11558060128811429No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG13610.11440378062045349No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC13590.11423566338221623No Hit
ACTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAAC13500.11347913581014858No Hit
CTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAAT13480.11331101857191131No Hit
GTCTAGTAGTTGCACATGTCAACTTAAAAGGTAAGTTATTCTTTTTAGCA13320.11196608066601325No Hit
ATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCT13220.11112549447482697No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC13110.11020084966452204No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG13100.11011679104540344No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC13030.10952838071157302No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA12820.10776314971008183No Hit
ATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCAATCTTTATAG12670.1065022704233024No Hit
GTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACAT12620.10608197732770924No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC12540.10540950837476024No Hit
GTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCA12520.10524139113652296No Hit
CAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTG12130.10196310499089646No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA12010.10095440156147291No Hit
CTCTTGTATTTTAATACCCTTATATTTACGCTGTATAGTTGAAACTATGG11980.10070222570411702No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA11950.10045004984676113No Hit
GTGTTACATAGCCAAGTGGCATTGTAACAAGAGTTTCATTTAGATCGTTA11940.1003659912276425No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC11910.10011381537028662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGG100.0070910305145.0145
ATTGCGG100.0070910305145.09
TTCCTAG6900.0120.8333362
TCCTAGG7000.0119.107153
TAGGTAG7500.0110.199996
AGAGCAG850.093.823535
CAGGTAT554.1654857E-1092.2727364
GTAGAGC900.088.6111153
AGGTAGT10750.076.883737
ATGCGCT1550.070.161296
TGCGCTA1600.067.968757
CCTAGGT12550.067.011954
TATGCGC1750.062.1428575
CGCTAGT1850.058.783789
AGGAGTA502.0103998E-458.07
TCGCCGG553.21925E-452.727276145
GGGCTTG553.21925E-452.7272761
GGAGTAG553.21925E-452.7272768
AGATCTG17850.050.364156
GATCTGT18950.047.058057
Produced by FastQC (version 0.11.5)