FastQCFastQC Report
Fri 3 Jul 2020
CG29K_l01_n02_44_100_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCG29K_l01_n02_44_100_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351447
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG50850.3762633680788074No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT48990.36250034222577726No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC40870.3024165949534092No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC36870.27281868989312935No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG35020.25912965880274996No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC30750.22753389515090122No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT30220.22361217273041417No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA28980.21443682216172738No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC27130.20074779107134796No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA26410.1954201681604976No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT23300.17240779697613No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA20470.15146727914598204No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC20240.14976539960501595No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT19740.14606566147248098No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG18950.14022007522307572No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT18340.13570639470138304No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA18120.13407850992306763No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT18040.13348655182186206No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT17820.13185866704354665No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC17230.12749297604715537No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA16980.12564310698088788No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT16730.1237932379146204No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC16360.12105543169654452No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC16240.12016749454473613No Hit
GACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAAC16140.11942754691822911No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC16000.11839162024111934No Hit
CAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACAC15740.11646775641220115No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG15430.11417391877002947No Hit
TTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTC15320.11335997638087175No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT15200.11247203922906336No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG15040.11128812302665218No Hit
GTTACCAGACACAAATGTGTTGTCTGTAGTAATGATTTGTGGTTCATAAA14760.10921626967243259No Hit
CTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAAC14740.10906828014713117No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT14650.1084023272832749No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT14630.1082543377579735No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG14540.1075883848941172No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC14520.1074403953688158No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC14490.10721841108086369No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT14400.1065524582170074No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA14400.1065524582170074No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTAC14390.1064784634543567No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT14310.1058865053531511No Hit
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAA14270.10559052630254831No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC14240.10536854201459621No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG14120.1044806048627878No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT14030.1038146519989315No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC13950.10322269389772593No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA13870.10263073579652032No Hit
TTTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATT13860.10255674103386962No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT13680.10122483530615703No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA13670.10115084054350633No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC13620.10078086673025284No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT13620.10078086673025284No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC13540.10018890862904724No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT13530.10011491386639652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAC100.007091136145.05
TCCTAGG5100.0112.303923
TTCCTAG5300.0108.066032
TAGGTAG5450.0105.091746
GTAGAGC406.6640336E-572.53
GGAGTAG903.6379788E-1272.499998
GAAGGAG903.6379788E-1272.499995
AGGAGTA955.456968E-1268.6842047
AGGTAGT8500.067.3823557
TGCCCCC350.003566774362.142857145
CCTAGTC350.003566774362.1428579
CAGGTAT2000.061.6254
GCCTAGC502.0104978E-458.0145
AGAGCAG502.0104978E-458.05
ACTGAAC400.006051344354.3753
GTTCCTA10550.054.28911
CCTAGGT10750.053.2790684
CAGACTG2900.052.55
GAGTAGC1258.913048E-1152.29
TATGCGC2400.051.3541685
Produced by FastQC (version 0.11.5)